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- PDB-4qb4: Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L -
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Open data
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Basic information
Entry | Database: PDB / ID: 4qb4 | ||||||
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Title | Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L | ||||||
![]() | Thermonuclease![]() | ||||||
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Function / homology | ![]() endonuclease activity, active with either ribo- or deoxyribonucleic acids and producing 3'-phosphomonoesters / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lor, P. / Janowska, K. / Sakon, J. / Stites, W.E. | ||||||
![]() | ![]() Title: Crystal Structure of Staphylococcal Nuclease mutant V23L/L25V/V66L Authors: Lor, P. / Janowska, K. / Sakon, J. / Stites, W.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 43.2 KB | Display | ![]() |
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PDB format | ![]() | 30.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 15551.081 Da / Num. of mol.: 1 / Fragment: UNP Residues 88-223 / Mutation: V23L, L25V, V66L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.7 % |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 0.6 M Ammonium sulfate, MPD 50-60%, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, temperature 277.0K |
-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU SATURN 92 / Detector: CCD / Date: Apr 19, 2011 / Details: mirrors |
Radiation | Monochromator: VariMax HF optics / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.6→19.409 Å / Num. all: 29329 / Num. obs: 15001 / % possible obs: 79.1 % / Observed criterion σ(I): 3 / Redundancy: 1.96 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 11.3 |
Reflection shell | Resolution: 1.6→1.66 Å / % possible all: 11.8 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.833 Å2
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Refinement step | Cycle: LAST / Resolution: 1.6→19.409 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.602→1.643 Å / Total num. of bins used: 20
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