+Open data
-Basic information
Entry | Database: PDB / ID: 4pug | ||||||
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Title | BolA1 from Arabidopsis thaliana | ||||||
Components | BolA like protein | ||||||
Keywords | DNA BINDING PROTEIN / stress-responsive protein / transcriptional regulator / morphogen / TRANSCRIPTION | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.998 Å | ||||||
Authors | Roret, T. / Didierjean, C. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2014 Title: Structural and Spectroscopic Insights into BolA-Glutaredoxin Complexes. Authors: Roret, T. / Tsan, P. / Couturier, J. / Zhang, B. / Johnson, M.K. / Rouhier, N. / Didierjean, C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4pug.cif.gz | 52.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4pug.ent.gz | 37.7 KB | Display | PDB format |
PDBx/mmJSON format | 4pug.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pu/4pug ftp://data.pdbj.org/pub/pdb/validation_reports/pu/4pug | HTTPS FTP |
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-Related structure data
Related structure data | 2mm9C 2mmaC 4puhC 4puiSC C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 11187.762 Da / Num. of mol.: 2 / Fragment: UNP residues 64-160 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: AT1G55805, At1g55805 / Production host: Escherichia coli (E. coli) / References: UniProt: Q682I1 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.11 % |
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Crystal grow | Temperature: 278 K / Method: oil microbatch method Details: 18% PEG4000, 6% 2-propanol, 6% 2.5 hexanediol, 4% xylitol and 0.07 M sodium acetate, Oil microbatch method, temperature 278K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM30A / Wavelength: 0.979733 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Feb 18, 2013 |
Radiation | Monochromator: SI (111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979733 Å / Relative weight: 1 |
Reflection | Resolution: 1.998→44.969 Å / Num. all: 12798 / Num. obs: 12798 / % possible obs: 99.8 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.6 % / Biso Wilson estimate: 18.3 Å2 / Rmerge(I) obs: 0.102 / Net I/σ(I): 11.3 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.351 / Mean I/σ(I) obs: 4.1 / Num. unique all: 1790 / % possible all: 98.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4PUI Resolution: 1.998→44.969 Å / SU ML: 0.25 / σ(F): 0 / σ(I): 0 / Phase error: 22.14 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 43.326 Å2 / ksol: 0.359 e/Å3 | |||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.998→44.969 Å
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Refine LS restraints |
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LS refinement shell |
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