+Open data
-Basic information
Entry | Database: PDB / ID: 4p0c | ||||||
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Title | Crystal Structure of NHERF2 PDZ1 Domain in Complex with LPA2 | ||||||
Components | Na(+)/H(+) exchange regulatory cofactor NHE-RF2/Lysophosphatidic acid receptor 2 chimeric protein | ||||||
Keywords | PROTEIN BINDING / PDZ / protein-protein interaction | ||||||
Function / homology | Function and homology information lysophosphatidic acid receptor activity / type 2 metabotropic glutamate receptor binding / Lysosphingolipid and LPA receptors / regulation of metabolic process / type 3 metabotropic glutamate receptor binding / plasma membrane => GO:0005886 / : / phosphatase binding / endomembrane system / protein-membrane adaptor activity ...lysophosphatidic acid receptor activity / type 2 metabotropic glutamate receptor binding / Lysosphingolipid and LPA receptors / regulation of metabolic process / type 3 metabotropic glutamate receptor binding / plasma membrane => GO:0005886 / : / phosphatase binding / endomembrane system / protein-membrane adaptor activity / protein localization to plasma membrane / G protein-coupled receptor activity / adenylate cyclase-activating G protein-coupled receptor signaling pathway / beta-catenin binding / G alpha (i) signalling events / positive regulation of cytosolic calcium ion concentration / G alpha (q) signalling events / protein-containing complex assembly / cadherin binding / apical plasma membrane / G protein-coupled receptor signaling pathway / signaling receptor binding / focal adhesion / lipid binding / cell surface / extracellular exosome / identical protein binding / nucleus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.339 Å | ||||||
Authors | Holcomb, J. / Jiang, Y. / Lu, G. / Trescott, L. / Brunzelle, J. / Sirinupong, N. / Li, C. / Naren, A. / Yang, Z. | ||||||
Citation | Journal: Biochem.Biophys.Res.Commun. / Year: 2014 Title: Structural insights into PDZ-mediated interaction of NHERF2 and LPA2, a cellular event implicated in CFTR channel regulation. Authors: Holcomb, J. / Jiang, Y. / Lu, G. / Trescott, L. / Brunzelle, J. / Sirinupong, N. / Li, C. / Naren, A.P. / Yang, Z. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4p0c.cif.gz | 68.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4p0c.ent.gz | 51.4 KB | Display | PDB format |
PDBx/mmJSON format | 4p0c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/p0/4p0c ftp://data.pdbj.org/pub/pdb/validation_reports/p0/4p0c | HTTPS FTP |
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-Related structure data
Related structure data | 20csS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 9801.165 Da / Num. of mol.: 1 Fragment: UNP Q15599 residues 9-90; UNP Q9HBW0 residues 347-351 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Plasmid: pSUMO / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q15599, UniProt: Q9HBW0 | ||
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#2: Chemical | ChemComp-SCN / | ||
#3: Chemical | #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 35.8 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / Details: 100 mM sodium acetate, 0.2 M ammonium acetate / PH range: 4.8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 1.12719 Å |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Jul 22, 2013 |
Radiation | Monochromator: Kohzu / Protocol: SINGLE WAVELENGTH / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.12719 Å / Relative weight: 1 |
Reflection | Resolution: 1.17→24.21 Å / Num. obs: 24020 / % possible obs: 95.8 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.045 / Net I/σ(I): 15.3 |
-Processing
Software | Name: PHENIX / Version: (phenix.refine: 1.8.4_1496) / Classification: refinement | |||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 20CS Resolution: 1.339→24.208 Å / SU ML: 0.13 / Cross valid method: THROUGHOUT / σ(F): 1.36 / Phase error: 18.08 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.339→24.208 Å
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Refine LS restraints |
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LS refinement shell |
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