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- PDB-4nq0: Structural insights into yeast histone chaperone Hif1: a scaffold... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4nq0 | ||||||
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Title | Structural insights into yeast histone chaperone Hif1: a scaffold protein recruiting protein complexes to core histones | ||||||
![]() | HAT1-interacting factor 1 | ||||||
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Function / homology | ![]() subtelomeric heterochromatin formation / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Liu, H. / Zhang, M. / He, W. / Zhu, Z. / Teng, M. / Gao, Y. / Niu, L. | ||||||
![]() | ![]() Title: Structural insights into yeast histone chaperone Hif1: a scaffold protein recruiting protein complexes to core histones Authors: Liu, H. / Zhang, M. / He, W. / Zhu, Z. / Teng, M. / Gao, Y. / Niu, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 115.4 KB | Display | ![]() |
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PDB format | ![]() | 89.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 44553.188 Da / Num. of mol.: 1 / Fragment: HAT1-interacting factor 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: ATCC 204508 / S288c / Gene: HIF1, YLL022C, L1205 / Production host: ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.75 Å3/Da / Density % sol: 29.9 % |
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Crystal grow![]() | Temperature: 287 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 0.1M sodium cacodylate, 15% PEG4000, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 20, 2011 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.1→50 Å / Num. all: 18938 / Num. obs: 18861 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 2 / Redundancy: 4.5 % / Rmerge(I) obs: 0.083 |
Reflection shell | Resolution: 2.1→2.14 Å / Redundancy: 4.5 % / Rmerge(I) obs: 0.656 / Mean I/σ(I) obs: 2.1 / Num. unique all: 917 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.268 Å2
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Refinement step | Cycle: LAST / Resolution: 2.1→41.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.155 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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