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- PDB-4nlz: Structure of human DNA polymerase beta complexed with nicked DNA ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4nlz | ||||||
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Title | Structure of human DNA polymerase beta complexed with nicked DNA containing a mismatched template 8BrG and incoming GTP | ||||||
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Function / homology | ![]() Resolution of AP sites via the single-nucleotide replacement pathway / immunoglobulin heavy chain V-D-J recombination / Resolution of AP sites via the multiple-nucleotide patch replacement pathway / Abasic sugar-phosphate removal via the single-nucleotide replacement pathway / APEX1-Independent Resolution of AP Sites via the Single Nucleotide Replacement Pathway / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Koag, M.-C. / Min, K. / Monzingo, A.F. / Lee, S. | ||||||
![]() | ![]() Title: Structural basis for promutagenicity of 8-halogenated Guanine. Authors: Koag, M.C. / Min, K. / Lee, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.5 KB | Display | ![]() |
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PDB format | ![]() | 71.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4m2yC ![]() 4m47C ![]() 4nlkC ![]() 4nlnC ![]() 4nm1C ![]() 4nm2C C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 1 types, 1 molecules A
#1: Protein | ![]() Mass: 37536.770 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: P06746, ![]() ![]() |
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-DNA chain , 3 types, 3 molecules TPD
#2: DNA chain | Mass: 4923.040 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: template |
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#3: DNA chain | Mass: 3414.234 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: up primer |
#4: DNA chain | Mass: 1536.035 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: down primer |
-Non-polymers , 4 types, 61 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/PO4.gif)
![](data/chem/img/HOH.gif)
#5: Chemical | ChemComp-MG / | ||||
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#6: Chemical | #7: Chemical | ChemComp-PO4 / | ![]() #8: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.93 % |
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Crystal grow![]() | Method: vapor diffusion, sitting drop / pH: 7.5 Details: 14-23% PEG3400, 350 mM sodium acetate, 50 mM imidazole, pH 7.5, VAPOR DIFFUSION, SITTING DROP |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Apr 24, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.683→52.839 Å / Num. all: 12692 / Num. obs: 12584 / % possible obs: 99.6 % / Observed criterion σ(F): 1 / Observed criterion σ(I): 1 |
Reflection shell | Highest resolution: 2.683 Å |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 66.64 Å2 / Biso mean: 22.2309 Å2 / Biso min: 3.34 Å2
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Refinement step | Cycle: LAST / Resolution: 2.683→52.839 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.683→2.752 Å / Total num. of bins used: 20
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