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- PDB-4mn8: Crystal structure of flg22 in complex with the FLS2 and BAK1 ecto... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4mn8 | |||||||||
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Title | Crystal structure of flg22 in complex with the FLS2 and BAK1 ectodomains | |||||||||
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![]() | TRANSFERASE/TRANSFERASE RECEPTOR / FLS2 / ![]() ![]() ![]() ![]() | |||||||||
Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Chai, J. / Sun, Y. / Han, Z. | |||||||||
![]() | ![]() Title: Structural basis for flg22-induced activation of the Arabidopsis FLS2-BAK1 immune complex. Authors: Sun, Y. / Li, L. / Macho, A.P. / Han, Z. / Hu, Z. / Zipfel, C. / Zhou, J.M. / Chai, J. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 394.3 KB | Display | ![]() |
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PDB format | ![]() | 323.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4mnaC ![]() 3rgzS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein , 2 types, 2 molecules AB
#1: Protein | Mass: 85524.281 Da / Num. of mol.: 1 / Fragment: FLS2-LRR UNP RESIDUES 25-800 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q9FL28, ![]() |
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#2: Protein | Mass: 24631.170 Da / Num. of mol.: 1 / Fragment: BAK1-LRR UNP RESIDUES 1-220 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: Q94F62, ![]() ![]() |
-Protein/peptide / Non-polymers , 2 types, 17 molecules C![](data/chem/img/SO4.gif)
![](data/chem/img/SO4.gif)
#3: Protein/peptide | Mass: 2275.502 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: This sequence is synthetic produce |
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#6: Chemical | ChemComp-SO4 / ![]() |
-Sugars , 2 types, 10 molecules ![](data/chem/img/NAG.gif)
#4: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose![]() Source method: isolated from a genetically manipulated source |
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#5: Sugar | ChemComp-NAG / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.52 Å3/Da / Density % sol: 72.81 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4 Details: 0.1M citirc acid pH 4.0, 2.5M ammonium sulfate, 8% (v/v) PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Jul 12, 2012 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.05→50 Å / Num. all: 38964 / Num. obs: 38653 / % possible obs: 99.2 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 1 / Redundancy: 3.5 % |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 3RGZ Resolution: 3.062→29.962 Å / SU ML: 0.4 / σ(F): 0 / Phase error: 31.09 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.062→29.962 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 14
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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