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Yorodumi- PDB-4lmw: Crystal structure of glutathione transferase GSTFuA3 from Phanero... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4lmw | ||||||
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Title | Crystal structure of glutathione transferase GSTFuA3 from Phanerochaete chrysosporium | ||||||
Components | Glutathione transferaseGlutathione S-transferase | ||||||
Keywords | TRANSFERASE / GST fold / glutathione transferase | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Phanerochaete chrysosporium (fungus) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.099 Å | ||||||
Authors | Didierjean, C. / Favier, F. / Prosper, P. | ||||||
Citation | Journal: Plos One / Year: 2013 Title: Diversification of fungal specific class a glutathione transferases in saprotrophic fungi. Authors: Mathieu, Y. / Prosper, P. / Favier, F. / Harvengt, L. / Didierjean, C. / Jacquot, J.P. / Morel-Rouhier, M. / Gelhaye, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4lmw.cif.gz | 117.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4lmw.ent.gz | 92.3 KB | Display | PDB format |
PDBx/mmJSON format | 4lmw.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lm/4lmw ftp://data.pdbj.org/pub/pdb/validation_reports/lm/4lmw | HTTPS FTP |
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-Related structure data
Related structure data | 4lmvC 4g19S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 28937.988 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Phanerochaete chrysosporium (fungus) / Plasmid: pSBET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) References: UniProt: A0A067XG73*PLUS, glutathione transferase | ||||
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#2: Chemical | ChemComp-FMT / #3: Water | ChemComp-HOH / | Sequence details | A SEQUENCE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4.26 Å3/Da / Density % sol: 71.13 % |
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Crystal grow | Temperature: 293 K / Method: microbatch under oil / pH: 4.6 Details: 0.1M Sodium acetate, 2M Sodium formate, pH 4.6, microbatch under oil, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.97911 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Mar 27, 2012 |
Radiation | Monochromator: Si 311 CHANNEL CUT / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97911 Å / Relative weight: 1 |
Reflection | Resolution: 2.099→45.164 Å / Num. all: 30431 / Num. obs: 30431 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 14.5 % / Biso Wilson estimate: 35.9 Å2 / Rmerge(I) obs: 0.064 / Net I/σ(I): 25.1 |
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 10.2 % / Rmerge(I) obs: 0.49 / Mean I/σ(I) obs: 4 / Num. unique all: 4218 / % possible all: 97.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4G19 Resolution: 2.099→45.164 Å / SU ML: 0.23 / σ(F): 1.35 / Phase error: 22.38 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.099→45.164 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 11
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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