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Open data
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Basic information
Entry | Database: PDB / ID: 4kma | ||||||
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Title | Crystal structure of Drosophila Suppressor of Fused | ||||||
![]() | GM14141p | ||||||
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Function / homology | ![]() negative regulation of nucleocytoplasmic transport / Hedgehog 'off' state / Degradation of GLI1 by the proteasome / GLI3 is processed to GLI3R by the proteasome / Hedgehog 'on' state / cytoplasmic sequestering of protein / molecular sequestering activity / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Zhang, Y. / Qi, X. / Zhang, Z. / Wu, G. | ||||||
![]() | ![]() Title: Structural insight into the mutual recognition and regulation between Suppressor of Fused and Gli/Ci. Authors: Zhang, Y. / Fu, L. / Qi, X. / Zhang, Z. / Xia, Y. / Jia, J. / Jiang, J. / Zhao, Y. / Wu, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 99.9 KB | Display | ![]() |
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PDB format | ![]() | 74.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4km8SC ![]() 4km9C ![]() 4kmdC ![]() 4kmhC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 56510.676 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
Sequence details | A SEQUENCE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. THE FIRST 30 RESIDUES ...A SEQUENCE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. THE FIRST 30 RESIDUES MSYYHHHHHH |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.32 Å3/Da / Density % sol: 47.09 % / Mosaicity: 12.629 ° |
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Crystal grow![]() | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 16% PEG 3350, 0.2M Magnesium acetate, vapor diffusion, hanging drop, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Detector | Detector: CCD / Date: Oct 1, 2012 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Radiation wavelength | Wavelength![]() | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection | Resolution: 2.68→50 Å / Num. obs: 14179 / % possible obs: 92.4 % / Redundancy: 9.2 % / Rmerge(I) obs: 0.137 / Χ2: 0.997 / Net I/σ(I): 9 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Reflection shell |
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-Phasing
Phasing![]() | Method: ![]() |
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Processing
Software |
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Refinement | Method to determine structure![]() ![]() Starting model: 4KM8 Resolution: 2.68→50 Å / Cor.coef. Fo:Fc: 0.895 / Cor.coef. Fo:Fc free: 0.836 / Occupancy max: 1 / Occupancy min: 0.6 / SU B: 15.362 / SU ML: 0.325 / Cross valid method: THROUGHOUT / σ(F): 0 / ESU R Free: 0.426 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 60.28 Å2 / Biso mean: 37.1516 Å2 / Biso min: 19.07 Å2
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Refinement step | Cycle: LAST / Resolution: 2.68→50 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.682→2.751 Å / Total num. of bins used: 20
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