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Open data
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Basic information
Entry | Database: PDB / ID: 4k7e | ||||||
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Title | Crystal structure of Junin virus nucleoprotein | ||||||
![]() | Nucleoprotein![]() | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of IKBKE activity / RNA-templated viral transcription / negative stranded viral RNA replication / helical viral capsid / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, Y.J. / Li, L. / Liu, X. / Dong, S.S. / Wang, W.M. / Huo, T. / Rao, Z.H. / Yang, C. | ||||||
![]() | ![]() Title: Crystal structure of Junin virus nucleoprotein Authors: Zhang, Y.J. / Li, L. / Liu, X. / Dong, S.S. / Wang, W.M. / Huo, T. / Guo, Y. / Rao, Z.H. / Yang, C. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 42 KB | Display | ![]() |
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PDB format | ![]() | 30.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 26667.738 Da / Num. of mol.: 1 / Fragment: C-TERMINAL DOMAIN, residues 341-564 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.02 Å3/Da / Density % sol: 39.16 % |
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Crystal grow![]() | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 8 Details: 0.1M Tris, 2.2M ammonium sulfate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 270 / Detector: CCD / Date: Dec 18, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.2→39.26 Å / Num. all: 11382 / Num. obs: 11370 / % possible obs: 99.8 % / Observed criterion σ(F): 3 / Observed criterion σ(I): 3 / Biso Wilson estimate: 32.11 Å2 |
Reflection shell | Resolution: 2.2→2.24 Å / Redundancy: 10.7 % / Rmerge(I) obs: 0.083 / Mean I/σ(I) obs: 53.5 / Num. unique all: 11382 / % possible all: 99.8 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.83 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 45.851 Å2 / ksol: 0.373 e/Å3 | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 88.72 Å2 / Biso mean: 41.8034 Å2 / Biso min: 20 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→39.256 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 4
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