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Open data
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Basic information
Entry | Database: PDB / ID: 4jz8 | ||||||
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Title | Carbamate kinase from Giardia lamblia bound to citric acid | ||||||
![]() | Carbamate kinase![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Lim, K. / Herzberg, O. | ||||||
![]() | ![]() Title: Crystal Structures of Carbamate Kinase from Giardia lamblia Bound with Citric Acid and AMP-PNP. Authors: Lim, K. / Kulakova, L. / Galkin, A. / Herzberg, O. #1: ![]() Title: X-ray structure and characterization of carbamate kinase from the human parasite Giardia lamblia. Authors: Galkin, A. / Kulakova, L. / Wu, R. / Nash, T.E. / Dunaway-Mariano, D. / Herzberg, O. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 254.5 KB | Display | ![]() |
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PDB format | ![]() | 205.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4jz7C ![]() 4jz9C ![]() 2we4S ![]() 3kzfS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 33975.219 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-CIT / ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.39 % |
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Crystal grow![]() | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 5 Details: 0.4 M ammonium citrate dibasic, pH 5.0, 21% PEG3350, crystal soaked in 2 mM inhibitor disulfiram (inhibitor did not bind but unit cell dimension b shrank by 6%), VAPOR DIFFUSION, HANGING DROP, temperature 295K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV++ / Detector: IMAGE PLATE / Date: Dec 13, 2011 / Details: mirrors |
Radiation | Monochromator: mirrors / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.1→46.3 Å / Num. all: 67363 / Num. obs: 67363 / % possible obs: 99.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 24 Å2 / Rmerge(I) obs: 0.092 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 2.1→2.15 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.322 / Mean I/σ(I) obs: 2.9 / Num. unique all: 3970 / % possible all: 97.5 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRIES 3KZF AND 2WE4 Resolution: 2.1→46.3 Å / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 31 Å2 | ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→46.3 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.15 Å
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