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Open data
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Basic information
Entry | Database: PDB / ID: 4jgg | ||||||
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Title | Crystal Structure of TesA | ||||||
![]() | Esterase TesA | ||||||
![]() | ![]() ![]() | ||||||
Function / homology | ![]() methyl indole-3-acetate esterase activity / ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kovacic, F. / Granzin, J. / Batra-Safferling, R. / Jaeger, K.-E. | ||||||
![]() | ![]() Title: Structural and Functional Characterisation of TesA - A Novel Lysophospholipase A from Pseudomonas aeruginosa. Authors: Kovacic, F. / Granzin, J. / Wilhelm, S. / Kojic-Prodic, B. / Batra-Safferling, R. / Jaeger, K.E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 82.9 KB | Display | ![]() |
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PDB format | ![]() | 61.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3hp4S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 21882.291 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 43.97 % |
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Crystal grow![]() | Temperature: 292 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: 20% PEG 3350, 50mM sodium-citrate, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 210 / Detector: CCD / Date: Nov 4, 2009 |
Radiation | Monochromator: Diamond (111), Ge(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.9→45.49 Å / Num. all: 29767 / Num. obs: 29422 / % possible obs: 98.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.2 % / Biso Wilson estimate: 13.45 Å2 / Rmerge(I) obs: 0.064 / Rsym value: 0.064 / Net I/σ(I): 13 |
Reflection shell | Resolution: 1.9→1.93 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.242 / Mean I/σ(I) obs: 4.7 / Num. unique all: 1485 / Rsym value: 0.242 / % possible all: 98.9 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3HP4 Resolution: 1.9→40.625 Å / SU ML: 0.26 / Cross valid method: THROUGHOUT / σ(F): 0 / Phase error: 28.3 / Stereochemistry target values: Engh & Huber
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Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.9→40.625 Å
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Refine LS restraints |
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LS refinement shell |
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