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Yorodumi- PDB-4iju: Crystal structure of 11b-HSD1 double mutant (L262R, F278E) in com... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4iju | ||||||
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Title | Crystal structure of 11b-HSD1 double mutant (L262R, F278E) in complex with (1S,4S)-4-[8-(2-fluorophenoxy)[1,2,4]triazolo[4,3-a]pyridin-3-yl]bicyclo[2.2.1]heptan-1-ol | ||||||
Components | Corticosteroid 11-beta-dehydrogenase isozyme 1 | ||||||
Keywords | OXIDOREDUCTASE/OXIDOREDUCTASE INHIBITOR / 11b-HSD1 / SDR / dehydrogenase / hydroxysteroid / inhibitor / OXIDOREDUCTASE-OXIDOREDUCTASE INHIBITOR complex | ||||||
Function / homology | Function and homology information 11beta-hydroxysteroid dehydrogenase / 11-beta-hydroxysteroid dehydrogenase (NADP+) activity / cortisol dehydrogenase activity / 7beta-hydroxysteroid dehydrogenase (NADP+) / 7-beta-hydroxysteroid dehydrogenase (NADP+) activity / Glucocorticoid biosynthesis / steroid catabolic process / Prednisone ADME / steroid binding / lung development ...11beta-hydroxysteroid dehydrogenase / 11-beta-hydroxysteroid dehydrogenase (NADP+) activity / cortisol dehydrogenase activity / 7beta-hydroxysteroid dehydrogenase (NADP+) / 7-beta-hydroxysteroid dehydrogenase (NADP+) activity / Glucocorticoid biosynthesis / steroid catabolic process / Prednisone ADME / steroid binding / lung development / NADP binding / intracellular membrane-bounded organelle / endoplasmic reticulum membrane / protein homodimerization activity / membrane Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.35 Å | ||||||
Authors | Sheriff, S. | ||||||
Citation | Journal: ACS Med Chem Lett / Year: 2014 Title: Optimization of 1,2,4-Triazolopyridines as Inhibitors of Human 11 beta-Hydroxysteroid Dehydrogenase Type 1 (11 beta-HSD-1). Authors: Li, J. / Kennedy, L.J. / Wang, H. / Li, J.J. / Walker, S.J. / Hong, Z. / O'Connor, S.P. / Nayeem, A. / Camac, D.M. / Morin, P.E. / Sheriff, S. / Wang, M. / Harper, T. / Golla, R. / Seethala, ...Authors: Li, J. / Kennedy, L.J. / Wang, H. / Li, J.J. / Walker, S.J. / Hong, Z. / O'Connor, S.P. / Nayeem, A. / Camac, D.M. / Morin, P.E. / Sheriff, S. / Wang, M. / Harper, T. / Golla, R. / Seethala, R. / Harrity, T. / Ponticiello, R.P. / Morgan, N.N. / Taylor, J.R. / Zebo, R. / Gordon, D.A. / Robl, J.A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4iju.cif.gz | 233.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4iju.ent.gz | 190.1 KB | Display | PDB format |
PDBx/mmJSON format | 4iju.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ij/4iju ftp://data.pdbj.org/pub/pdb/validation_reports/ij/4iju | HTTPS FTP |
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-Related structure data
Related structure data | 4ijvC 4ijwC 1xu7S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 31346.348 Da / Num. of mol.: 4 / Mutation: L262R, F278E Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: HSD11B1, HSD11, HSD11L / Plasmid: PET28 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: P28845, 11beta-hydroxysteroid dehydrogenase #2: Chemical | ChemComp-NAP / #3: Chemical | ChemComp-1EO / ( #4: Chemical | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.34 Å3/Da / Density % sol: 47.54 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 7.3 Details: 200 mM potassium formate, pH 7.3, 22% (W/V) PEG3350, 1.5 mM Zwittergent 3-12, vapor diffusion, hanging drop, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E / Wavelength: 1.54 / Wavelength: 1.54 Å |
Detector | Type: RIGAKU SATURN 92 / Detector: CCD / Date: Oct 2, 2006 / Details: MICROMAX CONFOCAL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 2.35→50 Å / Num. obs: 50073 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 6.4 % / Biso Wilson estimate: 32.69 Å2 / Rmerge(I) obs: 0.052 / Net I/σ(I): 32.9 |
Reflection shell | Resolution: 2.35→2.43 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.131 / Mean I/σ(I) obs: 9.8 / % possible all: 97.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1XU7 Resolution: 2.35→31.33 Å / Cor.coef. Fo:Fc: 0.937 / Cor.coef. Fo:Fc free: 0.9132 / Occupancy max: 1 / Occupancy min: 0.5 / SU R Cruickshank DPI: 0.352 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.388 / SU Rfree Blow DPI: 0.221 / SU Rfree Cruickshank DPI: 0.219
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Displacement parameters | Biso mean: 25.59 Å2
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Refine analyze | Luzzati coordinate error obs: 0.248 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.35→31.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.35→2.41 Å / Total num. of bins used: 20
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