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Yorodumi- PDB-4hll: Crystal structure of Artificial ankyrin repeat protein_Ank(GAG)1D4 -
+Open data
-Basic information
Entry | Database: PDB / ID: 4hll | ||||||
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Title | Crystal structure of Artificial ankyrin repeat protein_Ank(GAG)1D4 | ||||||
Components | Ankyrin(GAG)1D4 | ||||||
Keywords | PROTEIN BINDING / Ankyrin repeats | ||||||
Function / homology | Ankyrin repeat-containing domain / Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat / Alpha Horseshoe / Mainly Alpha Function and homology information | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Chuankhayan, P. / Nangola, S. / Minard, P. / Boulanger, P. / Hong, S.S. / Tayapiwatana, C. / Chen, C.-J. | ||||||
Citation | Journal: To be Published Title: Identification of Gag bioactive determinants specific to designed ankyrin and interfering in HIV-1 assembly Authors: Chuankhayan, P. / Nangola, S. / Minard, P. / Boulanger, P. / Hong, S.S. / Tayapiwatana, C. / Chen, C.-J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4hll.cif.gz | 41.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4hll.ent.gz | 28.7 KB | Display | PDB format |
PDBx/mmJSON format | 4hll.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hl/4hll ftp://data.pdbj.org/pub/pdb/validation_reports/hl/4hll | HTTPS FTP |
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-Related structure data
Related structure data | 3nocS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 18777.041 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) Description: THE SEQUENCE DERIVED FROM A PHAGE-DISPLAYED LIBRARY PRODUCED FOR E.COLI. Plasmid: pQE30 / Production host: Escherichia coli (E. coli) / Strain (production host): M15[pREP4] |
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#2: Water | ChemComp-HOH / |
Sequence details | THE SEQUENCE OF THIS PROTEIN WAS NOT AVAILABLE AT THE UNIPROT KNOWLEDGEBASE DATABASE (UNIPROTKB) AT ...THE SEQUENCE OF THIS PROTEIN WAS NOT AVAILABLE AT THE UNIPROT KNOWLEDGEB |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.07 Å3/Da / Density % sol: 40.64 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: 25% PEG 400, 5% PEG 3000, 10% glycerol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction |
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Diffraction source |
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Detector |
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Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 2.2→30 Å / Num. obs: 8038 / % possible obs: 99.3 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.4 % / Rsym value: 0.049 | |||||||||||||||
Reflection shell | Resolution: 2.2→2.3 Å / % possible all: 99.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3NOC Resolution: 2.2→30 Å / Occupancy max: 1 / Occupancy min: 1 / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 67.432 Å2 | ||||||||||||||||||||
Displacement parameters | Biso max: 134.65 Å2 / Biso mean: 36.5881 Å2 / Biso min: 14.5 Å2
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Refinement step | Cycle: LAST / Resolution: 2.2→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.2→2.3 Å | ||||||||||||||||||||
Xplor file |
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