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Open data
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Basic information
Entry | Database: PDB / ID: 4hkr | ||||||
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Title | Calcium release-activated calcium (CRAC) channel ORAI | ||||||
![]() | Calcium release-activated calcium channel protein 1 | ||||||
![]() | ![]() ![]() ![]() ![]() ![]() ![]() | ||||||
Function / homology | ![]() store-operated calcium entry / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Long, S.B. / Hou, X. | ||||||
![]() | ![]() Title: Crystal structure of the calcium release-activated calcium channel Orai. Authors: Hou, X. / Pedi, L. / Diver, M.M. / Long, S.B. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 73.9 KB | Display | ![]() |
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PDB format | ![]() | 53.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4hksSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Details | The biological assembly is a hexamer generated from the dimer in the asymmetric unit by the three fold crystallographic axis |
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Components
#1: Protein | Mass: 24347.395 Da / Num. of mol.: 2 / Fragment: UNP Residues 133-341 / Mutation: C224S, C283T, P276R, P277R Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-BA / | #3: Chemical | ChemComp-ZN / | #4: Chemical | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.81 Å3/Da / Density % sol: 56.16 % |
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Crystal grow![]() | Temperature: 290 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 26% PEG 400, 1 M Ammonium Formate, 100 mM MES pH 6.5. The crystal was soaked in: 50 % PEG 400, 1 M Ammonium Formate, 100 mM MES pH 6.5, 50 mM barium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Apr 22, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 3.35→40 Å / Num. all: 8116 / Num. obs: 8116 / % possible obs: 100 % / Observed criterion σ(I): -3 / Redundancy: 40.5 % / Biso Wilson estimate: 152 Å2 / Rsym value: 0.072 / Net I/σ(I): 59.7 |
Reflection shell | Resolution: 3.35→3.41 Å / Redundancy: 40.7 % / Mean I/σ(I) obs: 1.2 / Num. unique all: 386 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() ![]() Starting model: PDB ENTRY 4HKS Resolution: 3.3511→19.93 Å / SU ML: 0.31 / Isotropic thermal model: isotropic / σ(F): 0 / Phase error: 39.72 / Stereochemistry target values: Engh & Huber
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Solvent computation | Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 170 Å2 | ||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.3511→19.93 Å
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Refine LS restraints |
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LS refinement shell |
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