+Open data
-Basic information
Entry | Database: PDB / ID: 4guk | ||||||
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Title | New crystal form structure of human NCS1 | ||||||
Components | Neuronal calcium sensor 1 | ||||||
Keywords | PROTEIN BINDING / alpha / neuronal calcium sensor | ||||||
Function / homology | Function and homology information calcium sensitive guanylate cyclase activator activity / regulation of neuron projection development / voltage-gated calcium channel activity / postsynaptic density / axon / intracellular membrane-bounded organelle / dendrite / calcium ion binding / perinuclear region of cytoplasm / Golgi apparatus ...calcium sensitive guanylate cyclase activator activity / regulation of neuron projection development / voltage-gated calcium channel activity / postsynaptic density / axon / intracellular membrane-bounded organelle / dendrite / calcium ion binding / perinuclear region of cytoplasm / Golgi apparatus / plasma membrane / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.75 Å | ||||||
Authors | Fan, C. / Lolis, E. | ||||||
Citation | Journal: To be Published Title: new crystal form structure of human NCS1 Authors: Fan, C. / Lolis, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4guk.cif.gz | 318.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4guk.ent.gz | 255.9 KB | Display | PDB format |
PDBx/mmJSON format | 4guk.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gu/4guk ftp://data.pdbj.org/pub/pdb/validation_reports/gu/4guk | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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2 |
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3 |
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4 |
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Unit cell |
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-Components
-Protein , 1 types, 4 molecules ACBD
#1: Protein | Mass: 21902.668 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: 0.1mMIPTG 16C induction / Source: (gene. exp.) Homo sapiens (human) / Strain: human / Gene: FLUP, FREQ, NCS1 / Plasmid: pET21a(+) / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: P62166 |
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-Non-polymers , 8 types, 408 molecules
#2: Chemical | ChemComp-PG4 / #3: Chemical | ChemComp-EDO / #4: Chemical | ChemComp-CA / #5: Chemical | ChemComp-NA / #6: Chemical | ChemComp-P3G / | #7: Chemical | ChemComp-P2G / ( #8: Chemical | ChemComp-ALA / | #9: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.13 Å3/Da / Density % sol: 42.29 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: pH6.5, 0.2M NaAC, 30%PEG8000, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X25 / Wavelength: 1.1 Å |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Apr 28, 2011 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.1 Å / Relative weight: 1 |
Reflection | Resolution: 1.75→50 Å / Num. all: 75302 / Num. obs: 73721 / % possible obs: 97.9 % / Observed criterion σ(F): 2.7 / Observed criterion σ(I): 2.7 / Redundancy: 7.3 % / Rmerge(I) obs: 0.091 / Rsym value: 0.091 / Net I/σ(I): 11 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: human NCS1 Resolution: 1.75→50 Å / Isotropic thermal model: isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 1.75→50 Å
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Refine LS restraints |
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