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- PDB-4grp: Crystallographic and biological characterization of N- and C- ter... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4grp | ||||||
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Title | Crystallographic and biological characterization of N- and C- terminus mutants of human MIF | ||||||
![]() | Macrophage migration inhibitory factor![]() | ||||||
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Function / homology | ![]() positive regulation of prostaglandin secretion involved in immune response / positive regulation of myeloid leukocyte cytokine production involved in immune response / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Fan, C. / Lolis, E. | ||||||
![]() | ![]() Title: Crystallographic and biological characterization of N- and C- terminus mutants of human MIF Authors: Fan, C. / Lolis, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.2 KB | Display | ![]() |
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PDB format | ![]() | 132.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 12639.368 Da / Num. of mol.: 3 / Mutation: Pro1-Ala2 are insertions Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: P14174, ![]() ![]() #2: Chemical | ChemComp-SO4 / ![]() #3: Chemical | ![]() #4: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.06 Å3/Da / Density % sol: 40.42 % |
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Crystal grow![]() | Temperature: 293 K / pH: 5.6 Details: 1.6M ammonium sulfate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: PSI PILATUS 6M / Detector: PIXEL / Date: Dec 10, 2011 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.27→42.73 Å / Num. obs: 81548 / % possible obs: 97.6 % / Observed criterion σ(I): 0 / Redundancy: 8.2 % / Rmerge(I) obs: 0.09 / Rsym value: 0.09 / Net I/σ(I): 10.8 |
Reflection shell | Resolution: 1.34→1.7 Å / Redundancy: 0.46 % / Mean I/σ(I) obs: 1.8 / Rsym value: 0.459 / % possible all: 48.8 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: MIF Resolution: 1.27→41.84 Å / SU ML: 0.15 / σ(F): 1.37 / Phase error: 15.58 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 1.27→41.84 Å
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Refine LS restraints |
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LS refinement shell |
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