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- PDB-4g8t: Crystal structure of a glucarate dehydratase related protein, fro... -

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Basic information

Entry
Database: PDB / ID: 4g8t
TitleCrystal structure of a glucarate dehydratase related protein, from actinobacillus succinogenes, target EFI-502312, with sodium and sulfate bound, ordered loop
ComponentsGlucarate dehydratase
KeywordsLYASE / ENOLASE / putative glucarate dehydratase / enzyme function initiative / EFI / Structural Genomics
Function / homology
Function and homology information


glucarate dehydratase activity / D-glucarate catabolic process / glucarate dehydratase / magnesium ion binding
Similarity search - Function
Glucarate dehydratase / D-Glucarate dehydratase-like / Mandelate racemase DgoD-like / Mandelate racemase/muconate lactonizing enzyme, C-terminal / Mandelate racemase / muconate lactonizing enzyme, C-terminal domain / Enolase C-terminal domain-like / Enolase C-terminal domain-like / Enolase-like C-terminal domain / Enolase-like, N-terminal domain / Enolase-like, N-terminal ...Glucarate dehydratase / D-Glucarate dehydratase-like / Mandelate racemase DgoD-like / Mandelate racemase/muconate lactonizing enzyme, C-terminal / Mandelate racemase / muconate lactonizing enzyme, C-terminal domain / Enolase C-terminal domain-like / Enolase C-terminal domain-like / Enolase-like C-terminal domain / Enolase-like, N-terminal domain / Enolase-like, N-terminal / Enolase-like, C-terminal domain superfamily / Enolase-like; domain 1 / TIM Barrel / Alpha-Beta Barrel / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
2,3-DIHYDROXY-1,4-DITHIOBUTANE / (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL / Glucarate dehydratase
Similarity search - Component
Biological speciesActinobacillus succinogenes (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.7 Å
AuthorsVetting, M.W. / Toro, R. / Bhosle, R. / Al Obaidi, N.F. / Morisco, L.L. / Wasserman, S.R. / Sojitra, S. / Washington, E. / Scott Glenn, A. / Chowdhury, S. ...Vetting, M.W. / Toro, R. / Bhosle, R. / Al Obaidi, N.F. / Morisco, L.L. / Wasserman, S.R. / Sojitra, S. / Washington, E. / Scott Glenn, A. / Chowdhury, S. / Evans, B. / Hammonds, J. / Hillerich, B. / Love, J. / Seidel, R.D. / Imker, H.J. / Gerlt, J.A. / Almo, S.C. / Enzyme Function Initiative (EFI)
CitationJournal: TO BE PUBLISHED
Title: Crystal structure of a glucarate dehydratase related protein, from actinobacillus succinogenes, target efi-502312, with sodium and sulfate bound, ordered loop
Authors: Vetting, M.W. / Toro, R. / Bhosle, R. / Al Obaidi, N.F. / Morisco, L.L. / Wasserman, S.R. / Sojitra, S. / Washington, E. / Scott Glenn, A. / Chowdhury, S. / Evans, B. / Hammonds, J. / ...Authors: Vetting, M.W. / Toro, R. / Bhosle, R. / Al Obaidi, N.F. / Morisco, L.L. / Wasserman, S.R. / Sojitra, S. / Washington, E. / Scott Glenn, A. / Chowdhury, S. / Evans, B. / Hammonds, J. / Hillerich, B. / Love, J. / Seidel, R.D. / Imker, H.J. / Gerlt, J.A. / Almo, S.C. / Enzyme Function Initiative (EFI)
History
DepositionJul 23, 2012Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 15, 2012Provider: repository / Type: Initial release
Revision 1.1Sep 13, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Glucarate dehydratase
B: Glucarate dehydratase
C: Glucarate dehydratase
D: Glucarate dehydratase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)207,57923
Polymers205,8414
Non-polymers1,73819
Water35,5441973
1
A: Glucarate dehydratase
B: Glucarate dehydratase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)104,02014
Polymers102,9212
Non-polymers1,09912
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area5110 Å2
ΔGint-78 kcal/mol
Surface area30950 Å2
MethodPISA
2
C: Glucarate dehydratase
D: Glucarate dehydratase
hetero molecules


Theoretical massNumber of molelcules
Total (without water)103,5599
Polymers102,9212
Non-polymers6397
Water362
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area3890 Å2
ΔGint-81 kcal/mol
Surface area31130 Å2
MethodPISA
Unit cell
Length a, b, c (Å)110.837, 124.854, 139.093
Angle α, β, γ (deg.)90.00, 90.00, 90.00
Int Tables number19
Space group name H-MP212121

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Components

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Protein , 1 types, 4 molecules ABCD

#1: Protein
Glucarate dehydratase /


Mass: 51460.277 Da / Num. of mol.: 4
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Actinobacillus succinogenes (bacteria) / Strain: 130Z / Gene: Asuc_1847 / Plasmid: pET / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A6VQF1, glucarate dehydratase

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Non-polymers , 6 types, 1992 molecules

#2: Chemical
ChemComp-SO4 / SULFATE ION / Sulfate


Mass: 96.063 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: SO4
#3: Chemical
ChemComp-NA / SODIUM ION


Mass: 22.990 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Na
#4: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL / Glycerol


Mass: 92.094 Da / Num. of mol.: 7 / Source method: obtained synthetically / Formula: C3H8O3
#5: Chemical ChemComp-DTU / (2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL / Dithioerythritol


Mass: 154.251 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C4H10O2S2
#6: Chemical ChemComp-DTT / 2,3-DIHYDROXY-1,4-DITHIOBUTANE / 1,4-DITHIOTHREITOL / Dithiothreitol


Mass: 154.251 Da / Num. of mol.: 3 / Source method: obtained synthetically / Formula: C4H10O2S2
#7: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 1973 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.34 Å3/Da / Density % sol: 47.39 %
Crystal growTemperature: 298 K / Method: sitting drop vapor diffuction / pH: 7.5
Details: Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 0.1 M HEPES, 25% PEG4000); Cryoprotection (Reservoir, + ...Details: Protein (10 mM Hepes pH 7.5, 150 mM NaCl, 10% glycerol, 1 mM DTT, 5 mM MgCl); Reservoir (0.2 M Lithium Sulfate, 0.1 M Sodium Acetate, 0.1 M HEPES, 25% PEG4000); Cryoprotection (Reservoir, + 20% glycerol and 50 mM MgCl), sitting drop vapor diffuction, temperature 298K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 31-ID / Wavelength: 0.9793 Å
DetectorType: RAYONIX MX225HE / Detector: CCD / Date: Jan 1, 2012 / Details: MIRRORS
RadiationMonochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9793 Å / Relative weight: 1
ReflectionResolution: 1.7→139.093 Å / Num. all: 210738 / Num. obs: 210738 / % possible obs: 99.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 6.9 % / Rmerge(I) obs: 0.112 / Rsym value: 0.112 / Net I/σ(I): 9.2
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. measured allNum. unique allRsym value% possible all
1.7-1.796.40.5311.4195168303290.53199.1
1.79-1.96.50.3742186295287890.37499.5
1.9-2.036.60.2582.8179412272340.25899.8
2.03-2.196.80.193.6172835254130.1999.9
2.19-2.470.1624.1164855234230.16299.9
2.4-2.697.20.1564153039212580.156100
2.69-3.17.40.1394.5139326188320.139100
3.1-3.87.50.0897119861159990.089100
3.8-5.387.40.0591192764124960.059100
5.38-28.47770.043154869269650.04398.2

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Processing

Software
NameVersionClassificationNB
SCALA3.3.16data scaling
PHENIX1.7.3_928refinement
PDB_EXTRACT3.11data extraction
MOSFLMdata reduction
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 1EC7

1ec7


Resolution: 1.7→28.477 Å / Occupancy max: 1 / Occupancy min: 0.39 / FOM work R set: 0.8816 / SU ML: 0.17 / σ(F): 0 / σ(I): 0 / Phase error: 18.34 / Stereochemistry target values: ML
RfactorNum. reflection% reflectionSelection details
Rfree0.1791 10561 5.03 %RANDOM
Rwork0.1521 ---
all0.1535 210083 --
obs0.1535 210083 99.4 %-
Solvent computationShrinkage radii: 1.11 Å / VDW probe radii: 1.3 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 37.942 Å2 / ksol: 0.351 e/Å3
Displacement parametersBiso max: 124.53 Å2 / Biso mean: 22.9951 Å2 / Biso min: 8.2 Å2
Baniso -1Baniso -2Baniso -3
1-9.727 Å20 Å2-0 Å2
2---2.7479 Å2-0 Å2
3----6.979 Å2
Refinement stepCycle: LAST / Resolution: 1.7→28.477 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms13696 0 98 1973 15767
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.01114296
X-RAY DIFFRACTIONf_angle_d1.00519395
X-RAY DIFFRACTIONf_chiral_restr0.0712102
X-RAY DIFFRACTIONf_plane_restr0.0042539
X-RAY DIFFRACTIONf_dihedral_angle_d12.9295248
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 30

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
1.7-1.71930.24323430.21276499684299
1.7193-1.73950.26233520.2086578693099
1.7395-1.76070.24663320.19936568690099
1.7607-1.7830.2323590.19886554691399
1.783-1.80650.24023480.18866530687899
1.8065-1.83120.21263520.17736564691699
1.8312-1.85740.23153650.17596608697399
1.8574-1.88510.20553610.16936534689599
1.8851-1.91460.22063390.17016577691699
1.9146-1.94590.20343320.16186637696999
1.9459-1.97950.21123590.16156614697399
1.9795-2.01550.2043470.156366356982100
2.0155-2.05420.19943480.152166106958100
2.0542-2.09610.19593300.155266556985100
2.0961-2.14170.17963820.154766126994100
2.1417-2.19150.18753570.154366376994100
2.1915-2.24630.18913490.15366106959100
2.2463-2.3070.18973620.149866527014100
2.307-2.37490.17453560.148966296985100
2.3749-2.45150.18083220.148466857007100
2.4515-2.5390.17813510.15666737024100
2.539-2.64060.19763410.154566827023100
2.6406-2.76070.19293630.152466637026100
2.7607-2.90610.18163380.156667077045100
2.9061-3.0880.17733740.156667047078100
3.088-3.32610.15313520.142167187070100
3.3261-3.66020.1633680.13967557123100
3.6602-4.18840.13323820.12367627144100
4.1884-5.27150.14583430.128968717214100
5.2715-28.48090.16853540.16496999735399
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.706-0.15440.00971.0359-0.20620.6421-0.0317-0.0927-0.0210.14870.06590.24630.0747-0.2825-0.03640.1569-0.07520.03760.2318-0.02410.16845.072-12.54741.0493
20.7556-0.14160.14061.1794-0.4281.2981-0.059-0.09390.07750.21990.02790.0724-0.0028-0.14810.01760.1382-0.03040.04070.1969-0.05450.13287.34152.384840.6632
30.5315-0.42160.18330.6854-0.24020.684-0.027-0.03750.02740.05160.02350.05790.0795-0.11770.00060.1221-0.07750.00890.1547-0.02110.119812.8096-11.41633.947
40.86740.1361-0.15720.10540.13170.6145-0.0566-0.0723-0.3878-0.15890.0260.02240.6662-0.0507-0.21930.3495-0.1941-0.01270.0575-0.04060.168117.9173-35.079633.9352
51.05430.29480.06471.14760.31361.28560.00550.1148-0.1268-0.1659-0.0334-0.14030.25620.08680.04510.2283-0.00530.02190.1243-0.00260.165933.3645-26.126329.9523
60.59290.23960.07880.48620.02950.3536-0.01560.0221-0.0099-0.0080.00310.00610.1257-0.06730.00980.12-0.0328-0.00330.1125-0.01910.104325.7953-12.065128.8309
70.35270.1246-0.31290.4635-0.05480.2910.00390.0884-0.1125-0.11870.0090.23860.2195-0.30910.11520.1775-0.1667-0.02160.1993-0.03350.15147.8046-21.960928.5037
80.1493-0.22220.26910.4025-0.31820.60770.0087-0.074-0.06820.09210.06520.37860.1356-0.4159-0.1080.1476-0.15970.05540.389-0.00710.2184-2.2181-16.219438.3186
90.42410.0532-0.16340.7574-0.29330.82380.0446-0.1726-0.03930.2128-0.02340.01140.1812-0.0555-0.02150.2439-0.041-0.0050.1754-0.01050.126524.5745-25.346450.3015
100.68460.37280.00570.9107-0.13510.5115-0.02310.0660.1115-0.04080.04320.2256-0.0195-0.2302-0.00270.0973-0.0006-0.04510.1483-0.00370.125812.420620.23374.9481
110.57540.2967-0.08540.7977-0.18490.2723-0.10230.08880.101-0.18710.09430.25890.0622-0.2516-0.03380.1105-0.0162-0.05830.2265-0.01430.16376.124512.76415.0273
121.33950.9742-0.5860.9603-0.36620.249-0.13840.1857-0.2596-0.19310.1005-0.11120.1276-0.15650.00310.137-0.0254-0.02940.1564-0.03380.135616.61384.72676.4106
130.49040.1983-0.11150.4227-0.04920.6007-0.0078-0.02840.0785-0.01580.00720.1267-0.0352-0.1386-0.00210.08010.0151-0.01580.1308-0.02460.135414.528417.828117.1591
140.50840.0539-0.15390.6263-0.00641.2638-0.03190.02020.1940.0077-0.00570.0086-0.3444-0.00370.0290.2283-0.003-0.03580.1098-0.01150.198329.13336.116113.3271
150.67640.00510.09761.12730.39031.3715-0.025-0.04040.09030.0613-0.0214-0.1937-0.11260.14870.01690.0964-0.0134-0.03850.08740.00090.139640.928522.634917.0534
160.2971-0.1448-0.01680.5685-0.00560.4327-0.014-0.00460.0195-0.01340.0022-0.01090.0116-0.01350.0120.0742-0.0006-0.01250.0979-0.01760.108329.583411.638617.9736
170.51790.04760.30180.7255-0.19260.606-0.0296-0.13440.09480.10460.0290.2314-0.1549-0.2543-0.04720.09970.0348-0.00780.1584-0.03170.177615.691626.810418.826
180.48930.1263-0.41760.5866-0.49882.2554-0.03070.07230.146-0.06410.12020.3553-0.2581-0.55050.01150.12140.0366-0.07280.2772-0.00230.25374.30924.53519.4131
190.95450.1755-0.0081.1261-0.33871.2643-0.02420.15380.0656-0.3224-0.0222-0.0107-0.1296-0.01580.02950.1679-0.0061-0.01280.1257-0.00090.113532.186224.9537-3.3647
200.19410.05640.14940.49950.14180.79310.03510.1336-0.0284-0.3484-0.0009-0.2410.02180.3738-0.79920.22620.10980.17550.2362-0.00280.158371.2141-10.7498-0.5328
210.56080.1420.35390.7816-0.00911.7947-0.01460.14530.1823-0.2773-0.0048-0.1042-0.40190.2563-0.05190.27460.00220.13340.21090.03810.230273.76531.46727.992
220.73810.27490.51280.43920.37090.89330.00320.07640.1144-0.3625-0.0105-0.1393-0.05720.1035-0.03050.21620.06960.05330.12820.00390.134363.5774-11.48666.7107
230.10420.08440.16420.69210.04370.62010.10670.0785-0.1578-0.53070.02140.13150.4458-0.14240.19680.3710.11120.03680.018-0.08520.05448.6657-26.1489-3.4154
240.4946-0.2388-0.04650.56970.01440.36050.0230.01190.0129-0.1091-0.0433-0.02470.12560.04830.02370.14520.03810.00680.1299-0.01450.123250.6191-10.976612.012
250.72740.13-0.27750.38050.33590.51590.0756-0.0742-0.1969-0.1035-0.032-0.240.33160.28110.01750.28050.15840.05850.21330.00460.199264.6848-24.19434.8151
260.5466-0.02270.31060.74970.10610.7188-0.00570.1191-0.0048-0.40270.0068-0.16980.08120.2379-0.07350.29320.09950.08790.20080.01780.152462.0029-13.0848-7.7829
270.4057-0.4796-0.10441.11880.74531.31730.0472-0.0234-0.07390.1220.0129-0.09150.24480.1035-0.04110.08240.0039-0.03530.10140.02360.152969.2316-5.955641.1655
280.77060.07720.30470.81430.3010.84770.0247-0.05820.03470.0537-0.0409-0.0762-0.02070.01780.02380.0468-0.0052-0.00540.05330.01220.079658.62547.690343.7183
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'B' and (resseq 2:54)B2 - 54
2X-RAY DIFFRACTION2chain 'B' and (resseq 55:86)B55 - 86
3X-RAY DIFFRACTION3chain 'B' and (resseq 87:155)B87 - 155
4X-RAY DIFFRACTION4chain 'B' and (resseq 156:222)B156 - 222
5X-RAY DIFFRACTION5chain 'B' and (resseq 223:247)B223 - 247
6X-RAY DIFFRACTION6chain 'B' and (resseq 248:353)B248 - 353
7X-RAY DIFFRACTION7chain 'B' and (resseq 354:387)B354 - 387
8X-RAY DIFFRACTION8chain 'B' and (resseq 388:412)B388 - 412
9X-RAY DIFFRACTION9chain 'B' and (resseq 413:442)B413 - 442
10X-RAY DIFFRACTION10chain 'A' and (resseq 2:30)A2 - 30
11X-RAY DIFFRACTION11chain 'A' and (resseq 31:72)A31 - 72
12X-RAY DIFFRACTION12chain 'A' and (resseq 73:104)A73 - 104
13X-RAY DIFFRACTION13chain 'A' and (resseq 105:155)A105 - 155
14X-RAY DIFFRACTION14chain 'A' and (resseq 156:222)A156 - 222
15X-RAY DIFFRACTION15chain 'A' and (resseq 223:247)A223 - 247
16X-RAY DIFFRACTION16chain 'A' and (resseq 248:353)A248 - 353
17X-RAY DIFFRACTION17chain 'A' and (resseq 354:387)A354 - 387
18X-RAY DIFFRACTION18chain 'A' and (resseq 388:412)A388 - 412
19X-RAY DIFFRACTION19chain 'A' and (resseq 413:442)A413 - 442
20X-RAY DIFFRACTION20chain 'C' and (resseq 2:54)C2 - 54
21X-RAY DIFFRACTION21chain 'C' and (resseq 55:86)C55 - 86
22X-RAY DIFFRACTION22chain 'C' and (resseq 87:155)C87 - 155
23X-RAY DIFFRACTION23chain 'C' and (resseq 156:247)C156 - 247
24X-RAY DIFFRACTION24chain 'C' and (resseq 248:353)C248 - 353
25X-RAY DIFFRACTION25chain 'C' and (resseq 354:387)C354 - 387
26X-RAY DIFFRACTION26chain 'C' and (resseq 388:442)C388 - 442
27X-RAY DIFFRACTION27chain 'D' and (resseq 2:155)D2 - 155
28X-RAY DIFFRACTION28chain 'D' and (resseq 156:442)D156 - 442

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