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Open data
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Basic information
Entry | Database: PDB / ID: 4ees | ||||||
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Title | Crystal structure of iLOV | ||||||
![]() | Phototropin-2![]() | ||||||
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Function / homology | ![]() chloroplast relocation / negative regulation of anion channel activity by blue light / ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Hitomi, K. / Christie, J.M. / Arvai, A.S. / Hartfield, K.A. / Pratt, A.J. / Tainer, J.A. / Getzoff, E.D. | ||||||
![]() | ![]() Title: Structural Tuning of the Fluorescent Protein iLOV for Improved Photostability. Authors: Christie, J.M. / Hitomi, K. / Arvai, A.S. / Hartfield, K.A. / Mettlen, M. / Pratt, A.J. / Tainer, J.A. / Getzoff, E.D. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 38.5 KB | Display | ![]() |
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PDB format | ![]() | 25.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4eepC ![]() 4eerC ![]() 4eetC ![]() 4eeuC ![]() 2v1aS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 13278.843 Da / Num. of mol.: 1 / Fragment: LOV DOMAIN (UNP Residues 385-496) Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() References: UniProt: P93025, ![]() |
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#2: Chemical | ChemComp-FMN / ![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.92 Å3/Da / Density % sol: 36.08 % |
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Crystal grow![]() | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 4.8 Details: 37.5% MPEG 2K, 0.2 M imidazole malate, pH 4.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction source | Source: ![]() ![]() ![]() |
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Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Apr 15, 2008 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.2→50 Å / Num. obs: 10170 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 2V1A Resolution: 1.805→30 Å / Cor.coef. Fo:Fc: 0.958 / Cor.coef. Fo:Fc free: 0.939 / SU B: 3.196 / SU ML: 0.102 / Cross valid method: THROUGHOUT / ESU R: 0.156 / ESU R Free: 0.15 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 30.058 Å2
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Refinement step | Cycle: LAST / Resolution: 1.805→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.805→1.852 Å / Total num. of bins used: 20
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