+Open data
-Basic information
Entry | Database: PDB / ID: 4czt | ||||||
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Title | Crystal structure of the kinase domain of CIPK23 | ||||||
Components | CBL-INTERACTING SERINE/THREONINE-PROTEIN KINASE 23 | ||||||
Keywords | TRANSFERASE / POTASSIUM TRANSPORT / SNRK3 | ||||||
Function / homology | Function and homology information stomatal movement / regulation of stomatal movement / response to water deprivation / plastid / potassium ion import across plasma membrane / potassium channel activity / response to nutrient / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity ...stomatal movement / regulation of stomatal movement / response to water deprivation / plastid / potassium ion import across plasma membrane / potassium channel activity / response to nutrient / non-specific serine/threonine protein kinase / phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / signal transduction / ATP binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | ARABIDOPSIS THALIANA (thale cress) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.3 Å | ||||||
Authors | Chaves-Sanjuan, A. / Sanchez-Barrena, M.J. / Albert, A. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2014 Title: Structural Basis of the Regulatory Mechanism of the Plant Cipk Family of Protein Kinases Controlling Ion Homeostasis and Abiotic Stress Authors: Chaves-Sanjuan, A. / Sanchez-Barrena, M.J. / Gonzalez-Rubio, J.M. / Moreno, M. / Ragel, P. / Jimenez, M. / Pardo, J.M. / Martinez-Ripoll, M. / Quintero, F.J. / Albert, A. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4czt.cif.gz | 273.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4czt.ent.gz | 217.8 KB | Display | PDB format |
PDBx/mmJSON format | 4czt.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cz/4czt ftp://data.pdbj.org/pub/pdb/validation_reports/cz/4czt | HTTPS FTP |
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-Related structure data
Related structure data | 4czuC 4d28C 3h4jS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 51816.375 Da / Num. of mol.: 4 / Fragment: KINASE DOMAIN, RESIDUES 24-482 Source method: isolated from a genetically manipulated source Details: RESIDUES 19 TO 23 DO NOT CORRESPOND TO SNRK3. 23 AT1G30270 RESIDUES. THESE RESIDUES COMES FROM THE PGEX4T2 EXPRESSION VECTOR. Source: (gene. exp.) ARABIDOPSIS THALIANA (thale cress) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA PLYSS References: UniProt: Q93VD3, non-specific serine/threonine protein kinase #2: Chemical | ChemComp-CPS / #3: Chemical | ChemComp-SO4 / #4: Water | ChemComp-HOH / | Sequence details | RESIDUES 19 TO 23 DO NOT CORRESPOND TO SNRK3.23 AT1G30270 RESIDUES. THESE RESIDUES COMES FROM THE ...RESIDUES 19 TO 23 DO NOT CORRESPOND | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 49 % / Description: NONE |
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Crystal grow | Method: microbatch Details: 16 MM CHAPS 0.1 M HEPES PH 7.5, 3.5 M AMMONIUM SULFATE AS PRECIPITANT MIXED WITH THE PROTEIN AT 14 MG/ML USING MICROBATCH METHOD. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: ID14-4 / Wavelength: 0.9791 |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Oct 26, 2012 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9791 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→59.3 Å / Num. obs: 61816 / % possible obs: 99 % / Observed criterion σ(I): 3 / Redundancy: 13.2 % / Biso Wilson estimate: 32.88 Å2 / Rmerge(I) obs: 0.18 / Net I/σ(I): 12.8 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 12.4 % / Rmerge(I) obs: 1.15 / Mean I/σ(I) obs: 3 / % possible all: 99 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3H4J Resolution: 2.3→59.262 Å / SU ML: 0.27 / σ(F): 1.34 / Phase error: 23.44 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→59.262 Å
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Refine LS restraints |
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LS refinement shell |
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