[English] 日本語
Yorodumi- PDB-4b6v: The third member of the eIF4E family represses gene expression vi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 4b6v | ||||||
---|---|---|---|---|---|---|---|
Title | The third member of the eIF4E family represses gene expression via a novel mode of recognition of the methyl-7 guanosine cap moiety | ||||||
Components | EUKARYOTIC TRANSLATION INITIATION FACTOR 4E TYPE 3 | ||||||
Keywords | TRANSLATION / M7G CAP | ||||||
Function / homology | Function and homology information ISG15 antiviral mechanism / : / eukaryotic translation initiation factor 4F complex / RNA 7-methylguanosine cap binding / translation initiation factor activity Similarity search - Function | ||||||
Biological species | MUS MUSCULUS (house mouse) | ||||||
Method | SOLUTION NMR / XPLOR | ||||||
Authors | Osborne, M.J. / Volpon, L. / Kornblatt, J.A. / Culjkovic-Kraljcic, B. / Baguet, A. / Borden, K.L.B. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2013 Title: Eif4E3 Acts as a Tumor Suppressor by Utilizing an Atypical Mode of Methyl-7-Guanosine CAP Recognition. Authors: Osborne, M.J. / Volpon, L. / Kornblatt, J.A. / Culjkovic-Kraljacic, B. / Baguet, A. / Borden, K.L.B. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 4b6v.cif.gz | 621.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb4b6v.ent.gz | 524 KB | Display | PDB format |
PDBx/mmJSON format | 4b6v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/b6/4b6v ftp://data.pdbj.org/pub/pdb/validation_reports/b6/4b6v | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||
NMR ensembles |
|
-Components
#1: Protein | Mass: 22864.070 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) MUS MUSCULUS (house mouse) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9DBB5 |
---|
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
---|---|
NMR experiment | Type: NOESY |
NMR details | Text: NONE |
-Sample preparation
Details | Contents: 50MM PHOSPHATE, 100MM NACL, 100UM TCEP, NAN3, 7% D2O |
---|---|
Sample conditions | Ionic strength: 100 mM / pH: 7.3 / Pressure: 1.0 atm / Temperature: 293.0 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 800 MHz |
---|
-Processing
NMR software |
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method: XPLOR / Software ordinal: 1 | |||||||||
NMR ensemble | Conformer selection criteria: LEAST RESTRAINT VIOLATION / Conformers calculated total number: 100 / Conformers submitted total number: 10 |