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- PDB-4al7: Crystal structure of the Csy4-minimal crRNA complex -

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Basic information

Entry
Database: PDB / ID: 4al7
TitleCrystal structure of the Csy4-minimal crRNA complex
Components
  • 5'-R(*CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP*CP*AP*G)-3'
  • CSY4 ENDORIBONUCLEASE
KeywordsHYDROLASE/RNA / HYDROLASE-RNA COMPLEX / CRISPR
Function / homology
Function and homology information


maintenance of CRISPR repeat elements / endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / RNA binding
Similarity search - Function
CRISPR-associated endoribonuclease Cas6/Csy4 / CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST / CRISPR-associated endoribonuclease Cas6/Csy4, subtype I-F/YPEST superfamily / CRISPR-associated protein (Cas_Csy4) / Alpha-Beta Plaits / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
RNA / RNA (> 10) / CRISPR-associated endonuclease Cas6/Csy4
Similarity search - Component
Biological speciesPSEUDOMONAS AERUGINOSA (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.32 Å
AuthorsHaurwitz, R.E. / Sternberg, S.H. / Doudna, J.A.
CitationJournal: Embo J. / Year: 2012
Title: Csy4 Relies on an Unusual Catalytic Dyad to Position and Cleave Crispr RNA.
Authors: Haurwitz, R.E. / Sternberg, S.H. / Doudna, J.A.
History
DepositionMar 2, 2012Deposition site: PDBE / Processing site: PDBE
Revision 1.0Jun 13, 2012Provider: repository / Type: Initial release
Revision 1.1Jul 4, 2012Group: Other
Revision 1.2Dec 20, 2023Group: Data collection / Database references ...Data collection / Database references / Other / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_database_status / pdbx_initial_refinement_model
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_database_status.status_code_sf

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: CSY4 ENDORIBONUCLEASE
B: 5'-R(*CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP*CP*AP*G)-3'


Theoretical massNumber of molelcules
Total (without water)28,1802
Polymers28,1802
Non-polymers00
Water81145
1


  • Idetical with deposited unit
  • defined by author&software
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area1010 Å2
ΔGint-2 kcal/mol
Surface area12450 Å2
MethodPISA
Unit cell
Length a, b, c (Å)62.740, 47.280, 87.930
Angle α, β, γ (deg.)90.00, 106.72, 90.00
Int Tables number5
Space group name H-MC121
Components on special symmetry positions
IDModelComponents
11A-2028-

HOH

21A-2030-

HOH

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Components

#1: Protein CSY4 ENDORIBONUCLEASE


Mass: 21819.910 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) PSEUDOMONAS AERUGINOSA (bacteria) / Strain: UCBPP-PA14 / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): ROSETTA 2 / References: UniProt: Q02MM2
#2: RNA chain 5'-R(*CP*UP*GP*CP*CP*GP*UP*AP*UP*AP*GP*GP*CP*AP*G)-3' / CRRNA


Mass: 6359.815 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) PSEUDOMONAS AERUGINOSA (bacteria)
#3: Water ChemComp-HOH / water / Water


Mass: 18.015 Da / Num. of mol.: 45 / Source method: isolated from a natural source / Formula: H2O
Sequence detailsTHE UNIPROT DATABASE HAS AN INCORRECT START CODON ANNOTATION. THEREFORE, THE UNIPROT PROTEIN ...THE UNIPROT DATABASE HAS AN INCORRECT START CODON ANNOTATION. THEREFORE, THE UNIPROT PROTEIN SEQUENCE IS MISSING THE N-TERMINAL 20 AMINO ACIDS. THE CRRNA REPEAT IS DERIVED FROM THE CRISPR LOCUS LOCATED 2926520 TO 2927393.

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.37 Å3/Da / Density % sol: 52.24 % / Description: NONE
Crystal growpH: 5
Details: 21% PEG4000, 180 MM SODIUM CITRATE PH 5.0, 100 MM MAGNESIUM CHLORIDE, 2 MM AMMONIUM METAVANADATE

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: ALS / Beamline: 8.2.1 / Wavelength: 0.999982
DetectorType: ADSC QUANTUM 315r / Detector: CCD / Date: May 29, 2011
RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.999982 Å / Relative weight: 1
ReflectionResolution: 2.32→36.48 Å / Num. obs: 10773 / % possible obs: 99.4 % / Observed criterion σ(I): 1.99 / Redundancy: 4.9 % / Biso Wilson estimate: 38.68 Å2 / Rmerge(I) obs: 0.08 / Net I/σ(I): 13.5
Reflection shellResolution: 2.32→2.38 Å / Redundancy: 3.3 % / Rmerge(I) obs: 0.48 / Mean I/σ(I) obs: 2.49 / % possible all: 100

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Processing

Software
NameVersionClassification
PHENIX(PHENIX.REFINE)refinement
XDSdata reduction
XDSdata scaling
PHASERphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDB ENTRY 4AL5

4al5


Resolution: 2.32→36.476 Å / SU ML: 0.34 / σ(F): 1.99 / Phase error: 27.29 / Stereochemistry target values: ML
RfactorNum. reflection% reflection
Rfree0.2556 530 4.9 %
Rwork0.2023 --
obs0.205 10773 99.29 %
Solvent computationShrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 59.338 Å2 / ksol: 0.393 e/Å3
Displacement parametersBiso mean: 65.5 Å2
Baniso -1Baniso -2Baniso -3
1--3.1174 Å20 Å215.5705 Å2
2--7.5393 Å20 Å2
3---6.1462 Å2
Refinement stepCycle: LAST / Resolution: 2.32→36.476 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1175 306 0 45 1526
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.0151558
X-RAY DIFFRACTIONf_angle_d1.542188
X-RAY DIFFRACTIONf_dihedral_angle_d13.953600
X-RAY DIFFRACTIONf_chiral_restr0.088252
X-RAY DIFFRACTIONf_plane_restr0.008232
LS refinement shell
Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkRefine-ID% reflection obs (%)
2.32-2.55340.3091280.252522X-RAY DIFFRACTION100
2.5534-2.92280.29621310.22052556X-RAY DIFFRACTION99
2.9228-3.68180.24561300.17612570X-RAY DIFFRACTION99
3.6818-36.48050.24021410.20112595X-RAY DIFFRACTION99
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
10.1432-0.08590.05830.14430.03470.1197-0.19290.19420.16570.12120.19610.00960.10030.0401-0.03090.12-0.0637-0.00340.1206-0.01270.172812.91791.401129.809
20.09560.00330.01610.0157-0.00190.00620.03920.00680.0217-0.04310.06120.01650.0356-0.00740.12380.85970.03330.74471.2483-0.0174-0.078719.3219-4.52016.3738
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1CHAIN A AND (RESID -1:104 OR RESID 139:187)
2X-RAY DIFFRACTION2CHAIN B

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