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- PDB-4a56: Crystal structure of the type 2 secretion system pilotin from Kle... -
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Open data
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Basic information
Entry | Database: PDB / ID: 4a56 | ||||||
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Title | Crystal structure of the type 2 secretion system pilotin from Klebsiella Oxytoca | ||||||
![]() | PULLULANASE SECRETION PROTEIN PULS | ||||||
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Function / homology | ![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tosi, T. / Nickerson, N.N. / Mollica, L. / RingkjobingJensen, M. / Blackledge, M. / Baron, B. / England, P. / Pugsley, A.P. / Dessen, A. | ||||||
![]() | ![]() Title: Pilotin-Secretin Recognition in the Type II Secretion System of Klebsiella Oxytoca. Authors: Tosi, T. / Nickerson, N.N. / Mollica, L. / Jensen, M.R. / Blackledge, M. / Baron, B. / England, P. / Pugsley, A.P. / Dessen, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 47.1 KB | Display | ![]() |
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PDB format | ![]() | 37.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 10243.445 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: RESIDUES 30-122 / Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Chemical | ChemComp-MRD / (![]() |
#3: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.83 % / Description: NONE |
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Crystal grow![]() | pH: 7.5 / Details: 100 MM HEPES PH 7.5, 75% MPD |
-Data collection
Diffraction | Mean temperature: 230 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.24→4 Å / Num. obs: 47490 / % possible obs: 97.4 % / Observed criterion σ(I): 2 / Redundancy: 21 % / Biso Wilson estimate: 15 Å2 / Rmerge(I) obs: 0.02 / Rsym value: 0.05 / Net I/σ(I): 52 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: NONE Resolution: 1.24→54.84 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.955 / SU B: 1.201 / SU ML: 0.025 / Cross valid method: THROUGHOUT / ESU R: 0.045 / ESU R Free: 0.046 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 17.246 Å2
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Refinement step | Cycle: LAST / Resolution: 1.24→54.84 Å
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Refine LS restraints |
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