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Yorodumi- PDB-468d: CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-ME... -
+Open data
-Basic information
Entry | Database: PDB / ID: 468d | ||||||||||||||||||
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Title | CRYSTAL STRUCTURE AND IMPROVED ANTISENSE PROPERTIES OF 2'-O-(2-METHOXYETHYL)-RNA | ||||||||||||||||||
Components | RNA (5'-R(*Keywords | RNA / 2'-O-(2-METHOXYETHYL) RIBOSE / RNA DODECAMER DUPLEX / RIBONUCLEIC ACID | Function / homology | RNA / RNA (> 10) | Function and homology information Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.8 Å | Authors | Teplova, M. / Minasov, G. / Tereshko, V. / Inamati, G. / Cook, P.D. / Egli, M. | Citation | Journal: Nat.Struct.Biol. / Year: 1999 | Title: Crystal structure and improved antisense properties of 2'-O-(2-methoxyethyl)-RNA. Authors: Teplova, M. / Minasov, G. / Tereshko, V. / Inamati, G.B. / Cook, P.D. / Manoharan, M. / Egli, M. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 468d.cif.gz | 28.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb468d.ent.gz | 22.6 KB | Display | PDB format |
PDBx/mmJSON format | 468d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/68/468d ftp://data.pdbj.org/pub/pdb/validation_reports/68/468d | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: RNA chain | Mass: 4524.284 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: O2* OF EACH RIBOSE MODIFIED BY METHOXYETHYL #2: Chemical | ChemComp-MG / | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.84 Å3/Da / Density % sol: 33.03 % | |||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: DROPLETS CONTAINING 2MM OLIGONUCLEOTIDE, 50 MM MGCL2, 50 MM NA HEPES PH 7.5, 15% PEG 400, AGAINST 1 ML OF RESERVOIR SOLUTION CONTAINING 100 MM MGCL2, 100 MM NA HEPES PH 7.5, 30% PEG 400, ...Details: DROPLETS CONTAINING 2MM OLIGONUCLEOTIDE, 50 MM MGCL2, 50 MM NA HEPES PH 7.5, 15% PEG 400, AGAINST 1 ML OF RESERVOIR SOLUTION CONTAINING 100 MM MGCL2, 100 MM NA HEPES PH 7.5, 30% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 295K | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS | |||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 5ID-B / Wavelength: 0.978 |
Detector | Type: MARRESEARCH / Detector: CCD / Date: Feb 7, 1998 / Details: MIRRORS |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.978 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→20 Å / Num. all: 6889 / Num. obs: 6889 / % possible obs: 99.7 % / Observed criterion σ(I): 0 / Redundancy: 3.5 % / Rmerge(I) obs: 0.061 |
Reflection | *PLUS Num. measured all: 24219 |
-Processing
Software |
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Refinement | Resolution: 1.8→8 Å / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.8→8 Å
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Refine LS restraints |
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