HELIX DETERMINATION METHOD: DSSP OUTPUT MODIFIED BY AUTHOR
Remark 700
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AD" IN EACH CHAIN ON SHEET RECORDS BELOW ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AD" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 8-STRANDED BARREL THIS IS REPRESENTED BY A 9-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
Mass: 74747.906 Da / Num. of mol.: 1 / Fragment: RESIDUES 40-684 Source method: isolated from a genetically manipulated source Details: FRAGMENT CORRESPONDS TO RESIDUES 25-668 BASED ON NUMBERING STARTING AT SECOND UNIPROT INITIATION SITE Source: (gene. exp.) MUS MUSCULUS (house mouse) / Plasmid: PSECTAG2B / Cell line (production host): HEK293 / Production host: HOMO SAPIENS (human) / References: UniProt: P54818, galactosylceramidase
Mass: 18.015 Da / Num. of mol.: 139 / Source method: isolated from a natural source / Formula: H2O
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Details
Nonpolymer details
N-ACETYL-D-GLUCOSAMINE (NAG): THE LAST 3 NUMBERS MATCH THE ASN RESIDUE THEY ARE LINKED TO. THE ...N-ACETYL-D-GLUCOSAMINE (NAG): THE LAST 3 NUMBERS MATCH THE ASN RESIDUE THEY ARE LINKED TO. THE GLYCANS ARE THEN NUMBERED ACCORDING TO THEIR POSITION DISTANT FROM THE ATTACHED ASN.
Sequence details
TWO DIFFERENT START SITES ARE PRESENT IN MOUSE GALC, WE HAVE USED THE SECOND START SITE (TO MATCH ...TWO DIFFERENT START SITES ARE PRESENT IN MOUSE GALC, WE HAVE USED THE SECOND START SITE (TO MATCH THE LITERATURE) WHICH IS M17 IN THE UNIPROT ENTRY. THE FULL CONSTRUCT CONTAINED A SECRETION TAG (THAT WAS CLEAVED A HIS TAG AND A FACTOR XA SITE WHICH WERE NOT.
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 3.04 Å3/Da / Density % sol: 59.52 % / Description: NONE
Crystal grow
pH: 6.8 Details: 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.8, 34% (W/V) POLYETHYLENE GLYCOL 8000.
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