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Open data
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Basic information
Entry | Database: PDB / ID: 3w6j | ||||||
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Title | Crystal structure of ScpAB core complex | ||||||
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Function / homology | ![]() chromosome separation / ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kamada, K. / Hirano, T. | ||||||
![]() | ![]() Title: Molecular basis of SMC ATPase activation: role of internal structural changes of the regulatory subcomplex ScpAB Authors: Kamada, K. / Miyata, M. / Hirano, T. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 192.4 KB | Display | ![]() |
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PDB format | ![]() | 155.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20138.232 Da / Num. of mol.: 2 / Fragment: Unp residues 1-174 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: strain 10 / Gene: scpA / Plasmid: pET22b / Production host: ![]() ![]() ![]() #2: Protein | Mass: 20038.943 Da / Num. of mol.: 4 / Fragment: UNP resides 12-191 / Mutation: extra a.a. GPHM at Nter Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: strain 10 / Gene: scpB / Plasmid: modified pET28a / Production host: ![]() ![]() ![]() #3: Water | ChemComp-HOH / | ![]() Sequence details | A SEQUENCE DATABASE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. In THE ENTITY 2, GPHM ...A SEQUENCE DATABASE REFERENCE FOR THIS PROTEIN DOES NOT CURRENTLY EXIST. In THE ENTITY 2, GPHM AMINO ACIDS HAVE BEEN ADDED AT THE N TERMINAL. | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.27 Å3/Da / Density % sol: 71.22 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.2 Details: 0.49M NaH2PO4, 0.91M K2HPO4, pH 7.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jul 14, 2009 / Details: mirrors |
Radiation | Monochromator: Si 111 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.6→50 Å / Num. obs: 61981 / % possible obs: 98.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -3 / Redundancy: 3.6 % / Biso Wilson estimate: 66.659 Å2 / Rmerge(I) obs: 0.063 / Rsym value: 0.063 / Net I/σ(I): 19.793 |
Reflection shell | Resolution: 2.6→2.69 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.669 / Mean I/σ(I) obs: 1.625 / Num. unique all: 5598 / Rsym value: 0.669 / % possible all: 89.5 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Shrinkage radii: 0.86 Å / VDW probe radii: 1.1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 39.228 Å2 / ksol: 0.328 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters |
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Refinement step | Cycle: LAST / Resolution: 2.6→30.7 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 22
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