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- PDB-3vn4: Crystal structure of the exosite-containing fragment of human ADA... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3vn4 | |||||||||
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Title | Crystal structure of the exosite-containing fragment of human ADAMTS13 (P475S mutant) | |||||||||
![]() | A disintegrin and metalloproteinase with thrombospondin motifs 13 | |||||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Nakayama, D. / Akiyama, M. / Takeda, S. / Kokame, K. / Takagi, J. / Miyata, T. | |||||||||
![]() | ![]() Title: Structural analysis and biochemical studies of ADAMTS13 P475S mutant Authors: Nakayama, D. / Akiyama, M. / Takeda, S. / Kokame, K. / Takagi, J. / Miyata, T. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 2009 Title: Crystal structures of the noncatalytic domains of ADAMTS13 reveal multiple discontinuous exosites for von Willebrand factor Authors: Akiyama, M. / Takeda, S. / Kokame, K. / Takagi, J. / Miyata, T. #2: Journal: J.Thromb.Haemost. / Year: 2008 Title: ADAMTS13 P475S polymorphism causes a lowered enzymatic activity and urea lability in vitro Authors: Akiyama, M. / Kokame, K. / Miyata, T. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 166.4 KB | Display | ![]() |
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PDB format | ![]() | 130.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3ghmS S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
-Protein / Non-polymers , 2 types, 46 molecules A![](data/chem/img/HOH.gif)
![](data/chem/img/HOH.gif)
#1: Protein | Mass: 45339.148 Da / Num. of mol.: 1 / Fragment: DTCS DOMAINS, UNP residues 287-685 / Mutation: P475S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() |
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#6: Water | ChemComp-HOH / ![]() |
-Sugars , 4 types, 4 molecules ![](data/chem/img/NAG.gif)
![](data/chem/img/BMA.gif)
![](data/chem/img/BMA.gif)
#2: Polysaccharide | 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose![]() Source method: isolated from a genetically manipulated source |
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#3: Polysaccharide | beta-D-glucopyranose-(1-3)-alpha-L-fucopyranose![]() Source method: isolated from a genetically manipulated source |
#4: Sugar | ChemComp-NAG / ![]() |
#5: Sugar | ChemComp-BMA / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.21 Å3/Da / Density % sol: 61.66 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 20% PEG1500, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Feb 21, 2011 |
Radiation | Monochromator: Fixed exit Si (111) double crystal monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.8→50 Å / Num. all: 14407 / Num. obs: 14278 / % possible obs: 99.1 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 3.7 % / Rmerge(I) obs: 0.048 / Net I/σ(I): 20.3 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 3.7 % / Rmerge(I) obs: 0.31 / Mean I/σ(I) obs: 6.28 / Num. unique all: 1405 / % possible all: 99 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3GHM Resolution: 2.8→30.93 Å / Cor.coef. Fo:Fc: 0.926 / Cor.coef. Fo:Fc free: 0.899 / SU B: 30.293 / SU ML: 0.294 / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / ESU R Free: 0.374 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 62.369 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→30.93 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.8→2.87 Å / Total num. of bins used: 20
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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