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Open data
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Basic information
Entry | Database: PDB / ID: 3o2s | ||||||
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Title | Crystal structure of the human symplekin-Ssu72 complex | ||||||
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Function / homology | ![]() co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA polymerase II CTD heptapeptide repeat phosphatase activity / nuclear stress granule / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / RNA Polymerase II Transcription Termination / myosin phosphatase activity / termination of RNA polymerase II transcription ...co-transcriptional mRNA 3'-end processing, cleavage and polyadenylation pathway / RNA polymerase II CTD heptapeptide repeat phosphatase activity / nuclear stress granule / Processing of Intronless Pre-mRNAs / mRNA cleavage and polyadenylation specificity factor complex / mRNA 3'-end processing / Transport of Mature mRNA Derived from an Intronless Transcript / RNA Polymerase II Transcription Termination / myosin phosphatase activity / termination of RNA polymerase II transcription / : / protein-serine/threonine phosphatase / Processing of Capped Intron-Containing Pre-mRNA / RNA polymerase II transcribes snRNA genes / bicellular tight junction / negative regulation of protein binding / ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Tong, L. / Xiang, K. | ||||||
![]() | ![]() Title: Crystal structure of the human symplekin-Ssu72-CTD phosphopeptide complex. Authors: Xiang, K. / Nagaike, T. / Xiang, S. / Kilic, T. / Beh, M.M. / Manley, J.L. / Tong, L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 214.2 KB | Display | ![]() |
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PDB format | ![]() | 172 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 39646.957 Da / Num. of mol.: 1 / Fragment: N-terminal domain Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() |
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#2: Protein | ![]() Mass: 24774.852 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() References: UniProt: Q9NP77, protein-serine/threonine phosphatase |
#3: Chemical | ChemComp-PO4 / ![]() |
#4: Water | ChemComp-HOH / ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.59 Å3/Da / Density % sol: 52.49 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 0.32M ammonium sulfate, 26% (w/v) PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MAR CCD 165 mm / Detector: CCD / Date: Aug 21, 2009 / Details: MIRRORS |
Radiation | Monochromator: SI(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.5→30 Å / Num. obs: 24827 / % possible obs: 99.8 % / Redundancy: 4.1 % / Rmerge(I) obs: 0.067 / Net I/σ(I): 15.6908 |
Reflection shell | Resolution: 2.5→2.59 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.306 / Mean I/σ(I) obs: 3.055 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure![]() ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 40.874 Å2
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Refinement step | Cycle: LAST / Resolution: 2.5→30 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.634 Å / Total num. of bins used: 10
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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