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- PDB-3mui: Enzyme-Substrate interactions of IXT6, the intracellular xylanase... -
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Open data
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Basic information
Entry | Database: PDB / ID: 3mui | |||||||||
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Title | Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus. | |||||||||
![]() | Xylanase![]() | |||||||||
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Function / homology | ![]() ![]() ![]() Similarity search - Function | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Solomon, V. / Zolotnitsky, G. / Alhadeff, R. / Shoham, Y. / Shoham, G. | |||||||||
![]() | ![]() Title: Enzyme-Substrate interactions of IXT6, the intracellular xylanase of G. stearothermophilus. Authors: Solomon, V. / Zolotnitsky, G. / Alhadeff, R. / Shoham, Y. / Shoham, G. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 159.5 KB | Display | ![]() |
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PDB format | ![]() | 124.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3ms8C ![]() 3msdC ![]() 3msgC ![]() 3muaC ![]() 2q8xS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | ![]() Mass: 38637.816 Da / Num. of mol.: 2 / Mutation: E241A Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() Strain: T-6 / Gene: xynA2 / Plasmid: pET9d / Production host: ![]() ![]() ![]() References: UniProt: Q3YBZ9, UniProt: Q09LY9*PLUS, ![]() #2: Polysaccharide | #3: Chemical | #4: Chemical | ChemComp-GOL / ![]() #5: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.45 Å3/Da / Density % sol: 64.3 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.1M sodium cacodylate pH 6.5, 1.9M soduim acetate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4r / Detector: CCD / Date: Dec 5, 2002 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→19.97 Å / Num. obs: 91101 / % possible obs: 93.9 % / Redundancy: 2.98 % / Biso Wilson estimate: 20.8 Å2 / Rsym value: 0.056 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 1.8→1.83 Å / Redundancy: 2.74 % / Mean I/σ(I) obs: 2.12 / Num. unique all: 3971 / Rsym value: 0.412 / % possible all: 81.9 |
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Processing
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Refinement | Starting model: PDB ENTRY 2Q8X Resolution: 1.8→19.97 Å / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 30.8 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→19.97 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.83 Å / Rfactor Rfree error: 0.012
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