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Yorodumi- PDB-3msp: MOTILE MAJOR SPERM PROTEIN (MSP) OF ASCARIS SUUM, NMR, 20 STRUCTURES -
+Open data
-Basic information
Entry | Database: PDB / ID: 3msp | ||||||
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Title | MOTILE MAJOR SPERM PROTEIN (MSP) OF ASCARIS SUUM, NMR, 20 STRUCTURES | ||||||
Components | MAJOR SPERM PROTEIN | ||||||
Keywords | CELL MOTILITY PROTEIN / MAJOR SPERM PROTEIN / AMOEBOID MOTILITY / FILAMENTS / POLYMERIZATION | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Ascaris suum (pig roundworm) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Haaf, A. / Leclaire III, L. / Roberts, G. / Kent, H.M. / Roberts, T.M. / Stewart, M. / Neuhaus, D. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1998 Title: Solution structure of the motile major sperm protein (MSP) of Ascaris suum - evidence for two manganese binding sites and the possible role of divalent cations in filament formation. Authors: Haaf, A. / LeClaire 3rd., L. / Roberts, G. / Kent, H.M. / Roberts, T.M. / Stewart, M. / Neuhaus, D. #1: Journal: J.Mol.Biol. / Year: 1996 Title: 2.5 A Resolution Crystal Structure of the Motile Major Sperm Protein (Msp) of Ascaris Suum Authors: Bullock, T.L. / Roberts, T.M. / Stewart, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3msp.cif.gz | 1.5 MB | Display | PDBx/mmCIF format |
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PDB format | pdb3msp.ent.gz | 1.3 MB | Display | PDB format |
PDBx/mmJSON format | 3msp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ms/3msp ftp://data.pdbj.org/pub/pdb/validation_reports/ms/3msp | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 14278.107 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: EXPRESSED MATERIAL LACKS THE N-TERMINAL ACETYL GROUP THAT IS PRESENT IN WILD-TYPE MSP Source: (gene. exp.) Ascaris suum (pig roundworm) / Cell: SPERM CELL / Cell line: BL21 / Gene: ALPHA MSP / Plasmid: BL21 / Species (production host): Escherichia coli / Gene (production host): ALPHA MSP / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21 (DE3) / References: UniProt: P27439 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 10% D2O/90%H2O, 50 MM SODIUM PHOSPHATE, 3 MM SODIUM AZIDE |
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Sample conditions | pH: 4.5 / Pressure: 1 atm / Temperature: 308 K |
Crystal grow | *PLUS Method: other / Details: NMR |
-NMR measurement
NMR spectrometer |
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-Processing
Software |
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NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 / Details: REFINEMENT DETAILS GIVEN IN JRNL CITATION | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: LOW VIOLATION ENERGIES / Conformers calculated total number: 50 / Conformers submitted total number: 20 |