[English] 日本語
Yorodumi- PDB-3ldb: Structure of JMJD6 complexd with ALPHA-KETOGLUTARATE and Fab Fragment. -
+Open data
-Basic information
Entry | Database: PDB / ID: 3ldb | ||||||
---|---|---|---|---|---|---|---|
Title | Structure of JMJD6 complexd with ALPHA-KETOGLUTARATE and Fab Fragment. | ||||||
Components |
| ||||||
Keywords | IMMUNE SYSTEM / JMJ6D / ALPHA-KETOGLUTARATE / Fab Fragments | ||||||
Function / homology | Function and homology information peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine / histone H3R2 demethylase activity / peptidyl-lysine 5-dioxygenase activity / histone H4R3 demethylase activity / protein demethylase activity / recognition of apoptotic cell / negative regulation of protein homooligomerization / oxidative RNA demethylation / oxidative RNA demethylase activity / non-membrane-bounded organelle assembly ...peptidyl-lysine hydroxylation to 5-hydroxy-L-lysine / histone H3R2 demethylase activity / peptidyl-lysine 5-dioxygenase activity / histone H4R3 demethylase activity / protein demethylase activity / recognition of apoptotic cell / negative regulation of protein homooligomerization / oxidative RNA demethylation / oxidative RNA demethylase activity / non-membrane-bounded organelle assembly / Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor / macrophage activation / transcription regulator activator activity / regulation of mRNA splicing, via spliceosome / sprouting angiogenesis / erythrocyte development / Protein hydroxylation / P-TEFb complex binding / histone demethylase activity / phagocytosis / RNA splicing / kidney development / lung development / protein homooligomerization / HDMs demethylate histones / mRNA processing / retina development in camera-type eye / signaling receptor activity / T cell differentiation in thymus / heart development / single-stranded RNA binding / cell surface receptor signaling pathway / chromatin remodeling / iron ion binding / ribonucleoprotein complex / nucleolus / positive regulation of DNA-templated transcription / positive regulation of transcription by RNA polymerase II / RNA binding / nucleoplasm / identical protein binding / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) Cricetulus migratorius (Armenian hamster) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.7 Å | ||||||
Authors | Hong, X. / Zang, J. / White, J. / Kappler, J.W. / Wang, C. / Zhang, G. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.USA / Year: 2010 Title: Interaction of JMJD6 with single-stranded RNA. Authors: Hong, X. / Zang, J. / White, J. / Wang, C. / Pan, C.H. / Zhao, R. / Murphy, R.C. / Dai, S. / Henson, P. / Kappler, J.W. / Hagman, J. / Zhang, G. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3ldb.cif.gz | 169.4 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3ldb.ent.gz | 132.8 KB | Display | PDB format |
PDBx/mmJSON format | 3ldb.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ld/3ldb ftp://data.pdbj.org/pub/pdb/validation_reports/ld/3ldb | HTTPS FTP |
---|
-Related structure data
Related structure data | 3ld8SC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
-Protein , 1 types, 1 molecules A
#1: Protein | Mass: 39437.746 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: JMJD6, JMJD6 (1-403), KIAA0585, PTDSR / Plasmid: pET23b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 References: UniProt: Q6NYC1, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor |
---|
-Antibody , 2 types, 2 molecules BC
#2: Antibody | Mass: 24100.004 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cricetulus migratorius (Armenian hamster) Cell: hybridoma / Production host: Cricetulus migratorius (Armenian hamster) |
---|---|
#3: Antibody | Mass: 24007.662 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Cricetulus migratorius (Armenian hamster) Cell: hybridoma / Production host: Cricetulus migratorius (Armenian hamster) |
-Non-polymers , 5 types, 28 molecules
#4: Chemical | ChemComp-GOL / #5: Chemical | ChemComp-SO4 / #6: Chemical | ChemComp-AKG / | #7: Chemical | ChemComp-FE / | #8: Chemical | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 3 |
---|
-Sample preparation
Crystal | Density Matthews: 5.02 Å3/Da / Density % sol: 75.5 % |
---|---|
Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 5.6 Details: 0.1 M Bis(2-hydroxyethyl)-amino-tris(hydroxymethyl)-methane, 2.1 M Ammonium Sulphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction |
| |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Diffraction source |
| |||||||||||||||
Detector |
| |||||||||||||||
Radiation |
| |||||||||||||||
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 | |||||||||||||||
Reflection | Resolution: 2.61→99 Å / Num. all: 54702 / Num. obs: 48696 / % possible obs: 89 % / Biso Wilson estimate: 66.1 Å2 |
-Processing
Software |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB entries 3LD8, JMJD6, and Fab Fragment Resolution: 2.7→47.28 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 3487182.74 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Solvent model: FLAT MODEL / Bsol: 58.7914 Å2 / ksol: 0.35 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 83.5 Å2
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.7→47.28 Å
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints NCS | NCS model details: NONE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.7→2.87 Å / Rfactor Rfree error: 0.039 / Total num. of bins used: 6
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Xplor file |
|