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Yorodumi- PDB-3k9y: Crystal structure of rat mitochondrial P450 24A1 S57D in complex ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3k9y | ||||||
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Title | Crystal structure of rat mitochondrial P450 24A1 S57D in complex with CYMAL-5 | ||||||
Components | 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial | ||||||
Keywords | OXIDOREDUCTASE / MITOCHONDRIAL CYTOCHROME P450 / MONOTOPIC MEMBRANE PROTEIN / MONOOXYGENASE / VITAMIN D HORMONE METABOLISM / ADRENODOXIN | ||||||
Function / homology | Function and homology information vitamin D3 24-hydroxylase / 25-hydroxycholecalciferol-24-hydroxylase activity / 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity / 25-hydroxycholecalciferol-23-hydroxylase activity / vitamin D 25-hydroxylase activity / Vitamins / Vitamin D (calciferol) metabolism / 1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity / vitamin D 24-hydroxylase activity / vitamin D catabolic process ...vitamin D3 24-hydroxylase / 25-hydroxycholecalciferol-24-hydroxylase activity / 1-alpha,25-dihydroxyvitamin D3 24-hydroxylase activity / 25-hydroxycholecalciferol-23-hydroxylase activity / vitamin D 25-hydroxylase activity / Vitamins / Vitamin D (calciferol) metabolism / 1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity / vitamin D 24-hydroxylase activity / vitamin D catabolic process / vitamin D metabolic process / cellular response to vitamin D / response to vitamin D / osteoblast differentiation / iron ion binding / heme binding / mitochondrion Similarity search - Function | ||||||
Biological species | Rattus norvegicus (Norway rat) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Annalora, A.J. / Goodin, D.B. / Hong, W. / Zhang, Q. / Johnson, E.F. / Stout, C.D. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2010 Title: Crystal structure of CYP24A1, a mitochondrial cytochrome P450 involved in vitamin D metabolism. Authors: Annalora, A.J. / Goodin, D.B. / Hong, W.X. / Zhang, Q. / Johnson, E.F. / Stout, C.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3k9y.cif.gz | 188.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3k9y.ent.gz | 150.4 KB | Display | PDB format |
PDBx/mmJSON format | 3k9y.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/k9/3k9y ftp://data.pdbj.org/pub/pdb/validation_reports/k9/3k9y | HTTPS FTP |
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-Related structure data
Related structure data | 3k9vC 1tqnS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 55933.773 Da / Num. of mol.: 2 / Fragment: residues 34-514 / Mutation: S57D Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rattus norvegicus (Norway rat) / Gene: Cyp24, Cyp24a1 / Plasmid: pTRC99A / Production host: Escherichia coli (E. coli) / Strain (production host): DH5alpha-FIQ References: UniProt: Q09128, Oxidoreductases; Acting on paired donors, with incorporation or reduction of molecular oxygen; With NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor #2: Chemical | #3: Chemical | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.01 Å3/Da / Density % sol: 59.09 % |
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Crystal grow | Temperature: 290 K / Method: vapor diffusion, sitting drop / pH: 8.5 Details: 15% PEG 3350, 0.1 M Tris-HCl, 0.2M potassium thiocyanate, 0.05M CaCl, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
-Data collection
Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.97946 Å |
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Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Nov 7, 2007 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97946 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→91.67 Å / Num. all: 32727 / % possible obs: 97 % / Redundancy: 2.5 % / Biso Wilson estimate: 67.645 Å2 / Rmerge(I) obs: 0.166 / Rsym value: 0.166 / Net I/σ(I): 7.9 |
Reflection shell | Resolution: 2.8→2.87 Å / Redundancy: 2.5 % / Rmerge(I) obs: 1.332 / Mean I/σ(I) obs: 1.1 / Num. unique all: 1768 / Rsym value: 1.642 / % possible all: 70.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1TQN Resolution: 2.8→50 Å
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Displacement parameters | Biso mean: 77.151 Å2
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Refinement step | Cycle: LAST / Resolution: 2.8→50 Å
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Refine LS restraints |
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