+Open data
-Basic information
Entry | Database: PDB / ID: 3iga | ||||||
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Title | Potassium Channel KcsA-Fab complex in Li+ and K+ | ||||||
Components |
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Keywords | TRANSPORT PROTEIN / KcsA / Lithium / Lithium Block / Potassium Channel / Membrane Protein / Cell membrane / Ion transport / Ionic channel / Membrane / Transmembrane / Transport / Voltage-gated channel | ||||||
Function / homology | Function and homology information delayed rectifier potassium channel activity / voltage-gated potassium channel complex / identical protein binding Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.75 Å | ||||||
Authors | Thompson, A.N. / Ilsoo, K. / Panosian, T.D. / Iverson, T.M. / Allen, T.W. / Nimigean, C.M. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2009 Title: Mechanism of potassium-channel selectivity revealed by Na(+) and Li(+) binding sites within the KcsA pore. Authors: Thompson, A.N. / Kim, I. / Panosian, T.D. / Iverson, T.M. / Allen, T.W. / Nimigean, C.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3iga.cif.gz | 118.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3iga.ent.gz | 89.7 KB | Display | PDB format |
PDBx/mmJSON format | 3iga.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ig/3iga ftp://data.pdbj.org/pub/pdb/validation_reports/ig/3iga | HTTPS FTP |
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-Related structure data
Related structure data | 3gb7C 1k4dS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
-Protein , 1 types, 1 molecules C
#3: Protein | Mass: 13211.582 Da / Num. of mol.: 1 / Mutation: P2A, L90C Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Gene: kcsA, skc1 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 / References: UniProt: P0A334 |
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-Antibody , 2 types, 2 molecules AB
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
-Non-polymers , 3 types, 5 molecules
#4: Chemical | ChemComp-NI / |
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#5: Chemical | ChemComp-DGA / |
#6: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.83 Å3/Da / Density % sol: 67.87 % |
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-Data collection
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 21-ID-G / Wavelength: 0.979 Å |
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Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979 Å / Relative weight: 1 |
Reflection | Resolution: 2.75→30 Å / Num. obs: 21554 / Biso Wilson estimate: 79.4 Å2 |
-Processing
Software | Name: CNS / Version: 1.2 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1K4D Resolution: 2.75→27.54 Å / Rfactor Rfree error: 0.008 / Data cutoff high absF: 1961003.97 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.6577 Å2 / ksol: 0.3 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 79.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.75→27.54 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: NONE | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.75→2.85 Å / Rfactor Rfree error: 0.044 / Total num. of bins used: 10
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Xplor file |
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