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Yorodumi- PDB-3hui: Crystal Structure of the mutant A105R of [2Fe-2S] Ferredoxin in t... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3hui | ||||||
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Title | Crystal Structure of the mutant A105R of [2Fe-2S] Ferredoxin in the Class I CYP199A2 System from Rhodopseudomonas palustris | ||||||
Components | Ferredoxin | ||||||
Keywords | ELECTRON TRANSPORT / cytochrome P450 / ferredoxin / rhodopseudomonas palustris / electron transfer / Iron / Iron-sulfur / Metal-binding | ||||||
Function / homology | Function and homology information 2 iron, 2 sulfur cluster binding / electron transfer activity / metal ion binding Similarity search - Function | ||||||
Biological species | Rhodopseudomonas palustris (phototrophic) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.01 Å | ||||||
Authors | Bell, S.G. / Xu, F. / Rao, Z. / Wong, L.-L. | ||||||
Citation | Journal: J.Biol.Inorg.Chem. / Year: 2010 Title: Protein recognition in ferredoxin-P450 electron transfer in the class I CYP199A2 system from Rhodopseudomonas palustris Authors: Bell, S.G. / Xu, F. / Johnson, E.O. / Forward, I.M. / Bartlam, M. / Rao, Z. / Wong, L.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3hui.cif.gz | 36.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3hui.ent.gz | 23.4 KB | Display | PDB format |
PDBx/mmJSON format | 3hui.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hu/3hui ftp://data.pdbj.org/pub/pdb/validation_reports/hu/3hui | HTTPS FTP |
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-Related structure data
Related structure data | 1e9mS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 13757.359 Da / Num. of mol.: 1 / Mutation: A105R Source method: isolated from a genetically manipulated source Source: (gene. exp.) Rhodopseudomonas palustris (phototrophic) Plasmid: pET26 / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q6N2U2 |
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#2: Chemical | ChemComp-FES / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 1.95 Å3/Da / Density % sol: 36.86 % |
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Crystal grow | Temperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: 0.2M Ammonium Sulfate, 30% PEG8000, 0.1M Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU MICROMAX-007 / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Mar 20, 2006 |
Radiation | Monochromator: SAGITALLY FOCUSED SI(III) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.01→50 Å / Num. all: 7222 / Num. obs: 6641 / % possible obs: 92.6 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 2.4 % / Rmerge(I) obs: 0.076 |
Reflection shell | Resolution: 2.01→2.08 Å / Rmerge(I) obs: 0.14 / % possible all: 63.6 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1E9M Resolution: 2.01→50 Å / σ(F): 2 / σ(I): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.01→50 Å
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Refine LS restraints |
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