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Yorodumi- PDB-3f7v: KcsA Potassium channel in the open-inactivated state with 23 A op... -
+Open data
-Basic information
Entry | Database: PDB / ID: 3f7v | ||||||
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Title | KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 | ||||||
Components |
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Keywords | membrane protein/metal transport / KcsA / potassium channel / inactivation / open / Cell membrane / Ion transport / Ionic channel / Membrane / Transmembrane / Transport / Voltage-gated channel / membrane protein-metal transport COMPLEX | ||||||
Function / homology | Function and homology information delayed rectifier potassium channel activity / voltage-gated potassium channel complex / B cell differentiation / extracellular region / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Streptomyces lividans (bacteria) Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 3.2 Å | ||||||
Authors | Cuello, L.G. / Jogini, V. / Cortes, D.M. / Perozo, E. | ||||||
Citation | Journal: TO BE PUBLISHED Title: KcsA Potassium channel in the open-inactivated state with 23 A opening at T112 Authors: Cuello, L.G. / Jogini, V. / Cortes, D.M. / Perozo, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3f7v.cif.gz | 110.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3f7v.ent.gz | 85.5 KB | Display | PDB format |
PDBx/mmJSON format | 3f7v.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f7/3f7v ftp://data.pdbj.org/pub/pdb/validation_reports/f7/3f7v | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Antibody | Mass: 23411.242 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) |
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#2: Antibody | Mass: 23435.738 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Mus musculus (house mouse) / References: UniProt: P01837*PLUS |
#3: Protein | Mass: 10927.571 Da / Num. of mol.: 1 / Mutation: H25Q,L90C,R117Q,E120Q,R121Q,R122Q,H124Q Source method: isolated from a genetically manipulated source Source: (gene. exp.) Streptomyces lividans (bacteria) / Gene: kcsA, skc1 / Production host: Escherichia coli (E. coli) / Strain (production host): XL10-Gold / References: UniProt: P0A334 |
#4: Chemical |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.86 Å3/Da / Density % sol: 68.16 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop Details: 20-25% PEG400, 50mM magnesium acetate, 50mM sodium acetate, VAPOR DIFFUSION, SITTING DROP, temperature 298K PH range: 5.0-6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 24-ID-C / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Oct 12, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.2→40 Å / Num. all: 14716 / Num. obs: 13848 / % possible obs: 94.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 3.2→40 Å / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 3.2→40 Å
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Refine LS restraints |
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