+Open data
-Basic information
Entry | Database: PDB / ID: 3e8g | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal Structure of the the open NaK channel-Na+/Ca2+ complex | ||||||
Components | Potassium channel protein | ||||||
Keywords | MEMBRANE PROTEIN / non-selective cation channel / tetrameric cation channel family / 2-transmembrane helix channels / Ionic channel | ||||||
Function / homology | Function and homology information stabilization of membrane potential / potassium ion leak channel activity / outward rectifier potassium channel activity / membrane / identical protein binding / metal ion binding Similarity search - Function | ||||||
Biological species | Bacillus cereus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2 Å | ||||||
Authors | Jiang, Y. / Alam, A. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2009 Title: Structural analysis of ion selectivity in the NaK channel Authors: Alam, A. / Jiang, Y. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 3e8g.cif.gz | 50.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb3e8g.ent.gz | 36.8 KB | Display | PDB format |
PDBx/mmJSON format | 3e8g.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/e8/3e8g ftp://data.pdbj.org/pub/pdb/validation_reports/e8/3e8g | HTTPS FTP |
---|
-Related structure data
-Links
-Assembly
Deposited unit |
| |||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| |||||||||||||||||||||||||||||||||
2 |
| |||||||||||||||||||||||||||||||||
Unit cell |
| |||||||||||||||||||||||||||||||||
Components on special symmetry positions |
|
-Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 10706.538 Da / Num. of mol.: 2 / Fragment: transmembrane domain, residues 19-110 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus cereus (bacteria) / Plasmid: pQE60 / Production host: Escherichia coli (E. coli) / Strain (production host): SG13009 / References: UniProt: Q81HW2 |
---|
-Non-polymers , 5 types, 63 molecules
#2: Chemical | #3: Chemical | #4: Chemical | #5: Chemical | ChemComp-NA / #6: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 48.88 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 100mM Hepes, 55-70% (4S)-2-Methyl-2,4-pentanediol (MPD), 10mM CaCl2, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 19-ID |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2→50 Å / Num. obs: 13398 |
-Phasing
Phasing | Method: molecular replacement |
---|
-Processing
Software |
| ||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2→33.99 Å / Occupancy max: 1 / Occupancy min: 0.5 / σ(F): 0
| ||||||||||||||||||||||||
Solvent computation | Bsol: 113.782 Å2 | ||||||||||||||||||||||||
Displacement parameters | Biso max: 101.3 Å2 / Biso mean: 50.48 Å2 / Biso min: 23.96 Å2
| ||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2→33.99 Å
| ||||||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||||||
Xplor file |
|