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    - PDB-3c9v: C7 Symmetrized Structure of Unliganded GroEL at 4.7 Angstrom Reso... -

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    Basic information

    Entry
    Database: PDB / ID: 3c9v
    TitleC7 Symmetrized Structure of Unliganded GroEL at 4.7 Angstrom Resolution from CryoEM
    Descriptor60 kDa chaperonin
    KeywordsCHAPERONE / GroEL / ATP-binding / Cell cycle / Cell division / Chaperone / Nucleotide-binding / Phosphoprotein
    Specimen sourceEscherichia coli / bacteria /
    MethodElectron microscopy (4.7 A resolution)
    AuthorsLudtke, S.J. / Baker, M.L. / Chen, D.H. / Song, J.L. / Chuang, D. / Chiu, W.
    CitationStructure, 2008, 16, 441-448

    Structure, 2008, 16, 441-448 StrPapers
    De novo backbone trace of GroEL from single particle electron cryomicroscopy.
    Steven J Ludtke / Matthew L Baker / Dong-Hua Chen / Jiu-Li Song / David T Chuang / Wah Chiu

    DateDeposition: Feb 18, 2008 / Release: Sep 2, 2008 / Last modification: Jul 1, 2015

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    Assembly

    Deposited unit
    A: 60 kDa chaperonin
    B: 60 kDa chaperonin
    C: 60 kDa chaperonin
    D: 60 kDa chaperonin
    E: 60 kDa chaperonin
    F: 60 kDa chaperonin
    G: 60 kDa chaperonin
    H: 60 kDa chaperonin
    I: 60 kDa chaperonin
    J: 60 kDa chaperonin
    K: 60 kDa chaperonin
    L: 60 kDa chaperonin
    M: 60 kDa chaperonin
    N: 60 kDa chaperonin

    776 kDa, 14 molecules
    Theoretical massNumber of molelcules
    Total
    (without water)
    776,49514
    Polyers776,49514
    Non-polymers00
    Water0

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    #1idetical with deposited unit / defined by author / Symmetry operations: (identity)x1
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    Components

    #1polypeptide(L) / 60 kDa chaperonin / Protein Cpn60, groEL protein / Fragment: residues 2-527 / Source: Escherichia coli (gene. exp.) / References: UniProt: P0A6F5

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    Experimental details

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    Experiment

    ExperimentMethod: ELECTRON MICROSCOPY

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    Sample preparation

    Assembly of specimenName: GroEL / Aggregation state: PARTICLE
    ComponentName: GroEL / Details: 14-mer. Two back to back homo-heptameric rings.
    Buffer solutionName: 20 mM Tris.HCl, pH 7.5, 50 mM MgCl2
    Sample preparationpH: 7.5
    VitrificationDetails: ETHANE. Vitrobot, blot for 2 sec.

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    Electron microscopy imaging

    MicroscopyMicroscope model: JEOL 3200SFF / Date: 2005
    Electron gunElectron source: Field Emission Gun / Accelerating voltage: 300 kV / Electron dose: 36 e/A2 / Illumination mode: Flood Beam
    Electron lensMode: Bright Field / Nominal magnification: 60000 X / Nominal defocus max: 2300 nm / Nominal defocus min: 900 nm / Cs: 1.6 mm
    Specimen holderTemperature: 4 K / Tilt angle max: 0 deg. / Tilt angle min: 0 deg.
    CameraType: Kodak SO163 film
    RadiationDiffraction protocol: SINGLE WAVELENGTH / Monochromatic or laue m l: M / Scattering type: x-ray
    Radiation wavelengthRelative weight: 1

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    Processing

    3D reconstructionMethod: EMAN, single particle / Resolution: 4.7 A / Nominal pixel size: 1.06 A/pix / CTF correction method: per micrograph / Details: C7 symmetry, This entry contains CA atom only
    Number of atoms included #LASTProtein: 7364 / Nucleic acid: 0 / Ligand: 0 / Solvent: 0 / Total: 7364

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