+Open data
-Basic information
Entry | Database: PDB / ID: 3bck | ||||||
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Title | Crystal Structure of Staphylococcus aureus DsbA T153V | ||||||
Components | Disulfide bond protein ADisulfide | ||||||
Keywords | OXIDOREDUCTASE / thiol-disulfide oxidoreductase / redox protein / protein folding / redox active centre | ||||||
Function / homology | Function and homology information | ||||||
Biological species | Staphylococcus aureus (bacteria) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.95 Å | ||||||
Authors | Heras, B. / Thony-Meyer, L. / Martin, J.L. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2008 Title: Staphylococcus aureus DsbA Does Not Have a Destabilizing Disulfide: A NEW PARADIGM FOR BACTERIAL OXIDATIVE FOLDING Authors: Heras, B. / Kurz, M. / Jarrott, R. / Shouldice, S.R. / Frei, P. / Robin, G. / Cemazar, M. / Thony-Meyer, L. / Glockshuber, R. / Martin, J.L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3bck.cif.gz | 51.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3bck.ent.gz | 35.6 KB | Display | PDB format |
PDBx/mmJSON format | 3bck.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bc/3bck ftp://data.pdbj.org/pub/pdb/validation_reports/bc/3bck | HTTPS FTP |
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-Related structure data
Related structure data | 3bciSC 3bd2C S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 21808.006 Da / Num. of mol.: 1 / Fragment: residues in database 24-199 / Mutation: T153V Source method: isolated from a genetically manipulated source Source: (gene. exp.) Staphylococcus aureus (bacteria) / Strain: BB270 / Gene: AAG41993 / Plasmid: pET21a / Production host: Escherichia coli (E. coli) / Strain (production host): BL21 (DE3) pLysS / References: UniProt: Q9EYL5 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.18378 Å3/Da / Density % sol: 65.86 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion / Details: 28-30% PEG 3350, VAPOR DIFFUSION, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E+ SUPERBRIGHT / Wavelength: 1.54 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Oct 3, 2006 / Details: Osmic Confocal Max-Flux optics |
Radiation | Monochromator: Osmic Confocal MaxFlux / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 1.95→62.43 Å / Num. all: 19271 / Num. obs: 19271 / % possible obs: 96.8 % / Observed criterion σ(F): 0 / Redundancy: 5.06 % / Rmerge(I) obs: 0.066 / Net I/σ(I): 9.3 |
Reflection shell | Resolution: 1.95→2.02 Å / Redundancy: 4.07 % / Rmerge(I) obs: 0.523 / Mean I/σ(I) obs: 2.1 / Num. unique all: 1817 / % possible all: 92 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3BCI Resolution: 1.95→62.43 Å / Isotropic thermal model: anisotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 44.9 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.95→62.43 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.95→2.07 Å / Rfactor Rfree error: 0.035
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Xplor file |
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