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Open data
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Basic information
Entry | Database: PDB / ID: 3aqg | ||||||
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Title | Crystal structure of human pancreatic secretory protein ZG16b | ||||||
![]() | Zymogen granule protein 16 homolog B | ||||||
![]() | UNKNOWN FUNCTION / beta-prism fold | ||||||
Function / homology | ![]() retina homeostasis / ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kanagawa, M. / Satoh, T. / Nakano, Y. / Kojima-Aikawa, K. / Yamaguchi, Y. | ||||||
![]() | ![]() Title: Crystal structures of human secretory proteins ZG16p and ZG16b reveal a Jacalin-related beta-prism fold Authors: Kanagawa, M. / Satoh, T. / Ikeda, A. / Nakano, Y. / Yagi, H. / Kato, K. / Kojima-Aikawa, K. / Yamaguchi, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 66.1 KB | Display | ![]() |
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PDB format | ![]() | 48.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 3apaSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 17371.559 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() #2: Chemical | ![]() #3: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.99 Å3/Da / Density % sol: 38.16 % |
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Crystal grow![]() | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 8 Details: 0.4M ammonium phosphate monobasic, 5%(v/v) 1,4-dioxane, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: May 19, 2010 |
Radiation | Monochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.75→50 Å / Num. all: 7629 / Num. obs: 7619 / % possible obs: 99.9 % / Redundancy: 10.3 % / Rmerge(I) obs: 0.164 / Net I/σ(I): 14 |
Reflection shell | Resolution: 2.75→2.8 Å / Redundancy: 8.8 % / Rmerge(I) obs: 0.501 / Mean I/σ(I) obs: 3.5 / Num. unique all: 369 / % possible all: 99.7 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 3APA Resolution: 2.75→40 Å / Cor.coef. Fo:Fc: 0.899 / Cor.coef. Fo:Fc free: 0.836 / SU B: 17.287 / SU ML: 0.349 / Cross valid method: THROUGHOUT / ESU R Free: 0.436 / Stereochemistry target values: MAXIMUM LIKELIHOOD Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS U VALUES: REFINED INDIVIDUALLY
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 26.8 Å2
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Refinement step | Cycle: LAST / Resolution: 2.75→40 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.75→2.82 Å / Total num. of bins used: 20
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