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Yorodumi- PDB-3a5z: Crystal structure of Escherichia coli GenX in complex with elonga... -
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-Basic information
Entry | Database: PDB / ID: 3a5z | ||||||
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Title | Crystal structure of Escherichia coli GenX in complex with elongation factor P | ||||||
Components |
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Keywords | LIGASE / aminoacyl-tRNA synthetase paralog / Translation / tRNA / Lysyl-tRNA synthetase / Elongation Factor / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI / Aminoacyl-tRNA synthetase | ||||||
Function / homology | Function and homology information protein-lysine lysyltransferase activity / acid-ammonia (or amide) ligase activity / Ligases; Forming carbon-nitrogen bonds; Acid-ammonia (or amine) ligases (amide synthases) / lysine-tRNA ligase activity / lysyl-tRNA aminoacylation / cellular response to acidic pH / negative regulation of translational frameshifting / translational elongation / translation elongation factor activity / rescue of stalled ribosome ...protein-lysine lysyltransferase activity / acid-ammonia (or amide) ligase activity / Ligases; Forming carbon-nitrogen bonds; Acid-ammonia (or amine) ligases (amide synthases) / lysine-tRNA ligase activity / lysyl-tRNA aminoacylation / cellular response to acidic pH / negative regulation of translational frameshifting / translational elongation / translation elongation factor activity / rescue of stalled ribosome / ribosome binding / tRNA binding / protein homodimerization activity / ATP binding / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Escherichia coli (E. coli) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.5 Å | ||||||
Authors | Sumida, T. / Yanagisawa, T. / Ishii, R. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2010 Title: A paralog of lysyl-tRNA synthetase aminoacylates a conserved lysine residue in translation elongation factor P. Authors: Yanagisawa, T. / Sumida, T. / Ishii, R. / Takemoto, C. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 3a5z.cif.gz | 375.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb3a5z.ent.gz | 305.1 KB | Display | PDB format |
PDBx/mmJSON format | 3a5z.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a5/3a5z ftp://data.pdbj.org/pub/pdb/validation_reports/a5/3a5z | HTTPS FTP |
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-Related structure data
Related structure data | 3a5ySC 1uebS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data | |
Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 37299.285 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Strain: MC4100 / Gene: genX, ECs5136 / Plasmid: pET28c / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) References: UniProt: C3SGA2, UniProt: P0A8N7*PLUS, lysine-tRNA ligase #2: Protein | Mass: 20894.527 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: efp, ECs5128 / Plasmid: pET28c / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: C3SGD7, UniProt: P0A6N4*PLUS #3: Chemical | ChemComp-KAA / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.77 Å3/Da / Density % sol: 55.63 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 6.5 Details: Na/Cacodylate, Ammonium Sulfate, PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SPring-8 / Beamline: BL41XU / Wavelength: 1 Å |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 8, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.5→50 Å / Num. obs: 82592 / % possible obs: 93.8 % / Redundancy: 3.6 % / Biso Wilson estimate: 39 Å2 / Rsym value: 0.058 |
Reflection shell | Resolution: 2.5→2.59 Å / Rsym value: 0.357 / % possible all: 67.4 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3A5Y, 1UEB Resolution: 2.5→45.68 Å / Rfactor Rfree error: 0.004 / Data cutoff high absF: 904336.2 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 38.0015 Å2 / ksol: 0.323962 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 63.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→45.68 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file |
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