+Open data
-Basic information
Entry | Database: PDB / ID: 355d | ||||||||||||||||||||
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Title | THE B-DNA DODECAMER AT HIGH RESOLUTION | ||||||||||||||||||||
Components | DNA (5'-D(*Keywords | DNA / B-DNA / DOUBLE HELIX / SODIUM FORM | Function / homology | SPERMINE / DNA / DNA (> 10) | Function and homology information Biological species | synthetic construct (others) | Method | X-RAY DIFFRACTION / Resolution: 1.4 Å | Authors | Hu, L.D. / Shui, X. / McFail-Isom, L. / Williams, G.G. | Citation | Journal: Biochemistry / Year: 1998 | Title: The B-DNA dodecamer at high resolution reveals a spine of water on sodium. Authors: Shui, X. / McFail-Isom, L. / Hu, G.G. / Williams, L.D. History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 355d.cif.gz | 25 KB | Display | PDBx/mmCIF format |
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PDB format | pdb355d.ent.gz | 16.8 KB | Display | PDB format |
PDBx/mmJSON format | 355d.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/55/355d ftp://data.pdbj.org/pub/pdb/validation_reports/55/355d | HTTPS FTP |
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-Related structure data
Related structure data | 227dS S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: DNA chain | Mass: 3663.392 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #2: Chemical | ChemComp-MG / | #3: Chemical | ChemComp-SPM / | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.78 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6.5 Details: pH 6.50, VAPOR DIFFUSION, SITTING DROP, temperature 298.00K | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions |
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Crystal grow | *PLUS Temperature: 25 ℃ / pH: 6.5 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 136 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.5418 |
Detector | Type: ADSC / Detector: CCD |
Radiation | Monochromator: GRAPHITE / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Highest resolution: 1.38 Å / Num. all: 58307 / Num. obs: 11594 |
Reflection | *PLUS Highest resolution: 1.38 Å / Lowest resolution: 10 Å / Rmerge(I) obs: 0.044 |
Reflection shell | *PLUS Highest resolution: 1.38 Å / Lowest resolution: 1.48 Å / Rmerge(I) obs: 0.117 |
-Processing
Software |
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Refinement | Starting model: NDB ENTRY GDL036 (PDB ENTRY 227D) Resolution: 1.4→10 Å / σ(F): 0
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Refine Biso |
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Refinement step | Cycle: LAST / Resolution: 1.4→10 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.4→1.46 Å
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Software | *PLUS Name: X-PLOR / Version: 3.85 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 1.4 Å / Lowest resolution: 10 Å / σ(F): 0 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |