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Yorodumi- PDB-2yru: Solution structure of mouse Steroid receptor RNA activator 1 (SRA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2yru | ||||||
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Title | Solution structure of mouse Steroid receptor RNA activator 1 (SRA1) protein | ||||||
Components | Steroid receptor RNA activator 1 | ||||||
Keywords | APOPTOSIS / SRAP / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information steroid hormone aporeceptor complex / steroid receptor RNA activator RNA binding / SCAR complex / negative regulation of myoblast differentiation / intercellular bridge / cell leading edge / cellular response to estrogen stimulus / regulation of mitotic cell cycle / nuclear receptor coactivator activity / nuclear receptor binding ...steroid hormone aporeceptor complex / steroid receptor RNA activator RNA binding / SCAR complex / negative regulation of myoblast differentiation / intercellular bridge / cell leading edge / cellular response to estrogen stimulus / regulation of mitotic cell cycle / nuclear receptor coactivator activity / nuclear receptor binding / transcription coregulator activity / microtubule cytoskeleton / regulation of apoptotic process / transcription regulator complex / cell differentiation / transcription coactivator activity / ribonucleoprotein complex / apoptotic process / regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / DNA binding / nucleoplasm / nucleus / plasma membrane / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / torsion angle dynamics | ||||||
Authors | Nameki, N. / Saito, K. / Koshiba, S. / Kigawa, T. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: To be Published Title: Solution structure of mouse Steroid receptor RNA activator 1 (SRA1) protein Authors: Nameki, N. / Saito, K. / Koshiba, S. / Kigawa, T. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2yru.cif.gz | 709.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2yru.ent.gz | 615.9 KB | Display | PDB format |
PDBx/mmJSON format | 2yru.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/yr/2yru ftp://data.pdbj.org/pub/pdb/validation_reports/yr/2yru | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 13095.988 Da / Num. of mol.: 1 / Fragment: SRA1 domain, UNP residues 90-194 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Description: cell-free protein synthesis / Gene: Sra1 / Plasmid: P051025-17 / References: UniProt: Q80VJ2 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.2mM sample U-15N,13C; 20mM d-Tris-HCl; 100mM NaCl; 1mM d-DTT; 0.02% NaN3; 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | Ionic strength: 120mM / pH: 7.0 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics / Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations, target function Conformers calculated total number: 100 / Conformers submitted total number: 20 |