+Open data
-Basic information
Entry | Database: PDB / ID: 2wqo | ||||||
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Title | STRUCTURE OF NEK2 BOUND TO THE AMINOPYRIDINE CCT241950 | ||||||
Components | SERINE/THREONINE-PROTEIN KINASE NEK2 | ||||||
Keywords | TRANSFERASE / KINASE / NUCLEUS / MEIOSIS / MITOSIS / CYTOPLASM / ALTERNATIVE SPLICING / SERINE/THREONINE-PROTEIN KINASE / METAL-BINDING / PHOSPHOPROTEIN / NUCLEOTIDE-BINDING / MAGNESIUM / CELL CYCLE / ATP-BINDING / COILED COIL / POLYMORPHISM / CELL DIVISION | ||||||
Function / homology | Function and homology information negative regulation of centriole-centriole cohesion / centrosome separation / regulation of attachment of spindle microtubules to kinetochore / regulation of mitotic centrosome separation / regulation of mitotic nuclear division / positive regulation of telomere capping / blastocyst development / mitotic spindle assembly / spindle assembly / Loss of Nlp from mitotic centrosomes ...negative regulation of centriole-centriole cohesion / centrosome separation / regulation of attachment of spindle microtubules to kinetochore / regulation of mitotic centrosome separation / regulation of mitotic nuclear division / positive regulation of telomere capping / blastocyst development / mitotic spindle assembly / spindle assembly / Loss of Nlp from mitotic centrosomes / Loss of proteins required for interphase microtubule organization from the centrosome / Recruitment of mitotic centrosome proteins and complexes / positive regulation of telomerase activity / Recruitment of NuMA to mitotic centrosomes / Anchoring of the basal body to the plasma membrane / positive regulation of telomere maintenance via telomerase / APC-Cdc20 mediated degradation of Nek2A / AURKA Activation by TPX2 / meiotic cell cycle / condensed nuclear chromosome / chromosome segregation / kinetochore / spindle pole / Regulation of PLK1 Activity at G2/M Transition / mitotic cell cycle / midbody / protein phosphatase binding / microtubule / protein autophosphorylation / non-specific serine/threonine protein kinase / protein kinase activity / cell division / protein phosphorylation / protein serine kinase activity / protein serine/threonine kinase activity / centrosome / nucleolus / protein-containing complex / nucleoplasm / ATP binding / metal ion binding / nucleus / cytosol / cytoplasm Similarity search - Function | ||||||
Biological species | HOMO SAPIENS (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / OTHER / Resolution: 2.167 Å | ||||||
Authors | Mas-Droux, C. / Bayliss, R. | ||||||
Citation | Journal: Mol.Cell / Year: 2009 Title: An Autoinhibitory Tyrosine Motif in the Cell-Cycle- Regulated Nek7 Kinase is Released Through Binding of Nek9. Authors: Richards, M.W. / O'Regan, L. / Mas-Droux, C. / Blot, J.M. / Cheung, J. / Hoelder, S. / Fry, A.M. / Bayliss, R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2wqo.cif.gz | 66 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2wqo.ent.gz | 48.5 KB | Display | PDB format |
PDBx/mmJSON format | 2wqo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wq/2wqo ftp://data.pdbj.org/pub/pdb/validation_reports/wq/2wqo | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 32662.479 Da / Num. of mol.: 1 / Fragment: CATALYTIC DOMAIN, RESIDUES 1-271 / Source method: isolated from a natural source / Source: (natural) HOMO SAPIENS (human) References: UniProt: P51955, non-specific serine/threonine protein kinase | ||||
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#2: Chemical | ChemComp-VGK / | ||||
#3: Chemical | #4: Water | ChemComp-HOH / | Sequence details | C-TERMINAL LEHHHHHH AFFINITY TAG FROM VECTOR SEQUENCE | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.22 Å3/Da / Density % sol: 61.78 % / Description: NONE |
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Crystal grow | Details: 2-10% PEG8000, 50MM TRIS PH 8.5, |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I02 / Wavelength: 0.9796 |
Detector | Type: ADSC CCD / Detector: CCD |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 2.17→57.8 Å / Num. obs: 17404 / % possible obs: 99.7 % / Observed criterion σ(I): 2 / Redundancy: 3.4 % / Biso Wilson estimate: 24.21 Å2 / Rmerge(I) obs: 0.09 / Net I/σ(I): 10.3 |
Reflection shell | Resolution: 2.17→2.28 Å / Redundancy: 3.4 % / Rmerge(I) obs: 0.38 / Mean I/σ(I) obs: 3.4 / % possible all: 99.9 |
-Processing
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 2.167→57.84 Å / SU ML: 0.3 / σ(F): 0.02 / Phase error: 24.42 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 52.63 Å2 / ksol: 0.351 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 35.52 Å2
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Refinement step | Cycle: LAST / Resolution: 2.167→57.84 Å
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Refine LS restraints |
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LS refinement shell |
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