- PDB-2w9r: Structural basis of N-end rule substrate recognition in Escherich... -
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基本情報
登録情報
データベース: PDB / ID: 2w9r
タイトル
Structural basis of N-end rule substrate recognition in Escherichia coli by the ClpAP adaptor protein ClpS
要素
ATP-DEPENDENT CLP PROTEASE ADAPTER PROTEIN CLPS
DNA PROTECTION DURING STARVATION PROTEIN
キーワード
CHAPERONE (シャペロン) / ADAPTOR PROTEIN / DNA CONDENSATION / IRON (鉄) / CLPS / CLPA / CYTOPLASM (細胞質) / N-END RULE / DNA-BINDING / IRON STORAGE / METAL-BINDING / OXIDOREDUCTASE (酸化還元酵素)
機能・相同性
機能・相同性情報
DnaA-Dps complex / 酸化還元酵素; 金属イオンを酸化する / oxidoreductase activity, acting on metal ions / 核様体 / chromosome condensation / molecular function inhibitor activity / response to starvation / negative regulation of DNA-templated DNA replication initiation / ferric iron binding / protein catabolic process ...DnaA-Dps complex / 酸化還元酵素; 金属イオンを酸化する / oxidoreductase activity, acting on metal ions / 核様体 / chromosome condensation / molecular function inhibitor activity / response to starvation / negative regulation of DNA-templated DNA replication initiation / ferric iron binding / protein catabolic process / protein-folding chaperone binding / response to heat / intracellular iron ion homeostasis / タンパク質分解 / DNA binding / 生体膜 / identical protein binding / 細胞質 類似検索 - 分子機能
DNA protection during starvation protein, gammaproteobacteria / Ribosomal protein L7/L12, C-terminal domain/Adaptor protein ClpS / ATP-dependent Clp protease adaptor protein ClpS / Adaptor protein ClpS, core / ATP-dependent Clp protease adaptor protein ClpS / Dps protein family signature 2. / Dps protein family signature 1. / DNA-binding protein Dps, conserved site / DNA-binding protein Dps / Ribosomal Protein L30; Chain: A, ...DNA protection during starvation protein, gammaproteobacteria / Ribosomal protein L7/L12, C-terminal domain/Adaptor protein ClpS / ATP-dependent Clp protease adaptor protein ClpS / Adaptor protein ClpS, core / ATP-dependent Clp protease adaptor protein ClpS / Dps protein family signature 2. / Dps protein family signature 1. / DNA-binding protein Dps, conserved site / DNA-binding protein Dps / Ribosomal Protein L30; Chain: A, / Ribosomal protein L7/L12, C-terminal/adaptor protein ClpS-like / Ferritin/DPS protein domain / Ferritin-like domain / Ferritin-like / Ferritin-like superfamily / 2-Layer Sandwich / Alpha Beta 類似検索 - ドメイン・相同性
ATP-dependent Clp protease adapter protein ClpS / DNA protection during starvation protein 類似検索 - 構成要素
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.97 Å / 相対比: 1
反射
解像度: 1.7→30 Å / Num. obs: 11540 / % possible obs: 91.1 % / Observed criterion σ(I): 4 / 冗長度: 2.1 % / Rmerge(I) obs: 0.04 / Net I/σ(I): 12.4
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解析
ソフトウェア
名称
バージョン
分類
REFMAC
5.2.0019
精密化
XDS
データ削減
XDS
データスケーリング
精密化
構造決定の手法: その他 開始モデル: NONE 解像度: 1.7→25 Å / Cor.coef. Fo:Fc: 0.932 / Cor.coef. Fo:Fc free: 0.917 / SU B: 6.186 / SU ML: 0.094 / 交差検証法: THROUGHOUT / ESU R: 0.236 / ESU R Free: 0.128 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
Rfactor
反射数
%反射
Selection details
Rfree
0.254
574
5 %
RANDOM
Rwork
0.226
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obs
0.228
10925
100 %
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溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: MASK