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- PDB-2uwc: Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2 -
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Open data
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Basic information
Entry | Database: PDB / ID: 2uwc | ||||||
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Title | Crystal structure of Nasturtium xyloglucan hydrolase isoform NXG2 | ||||||
![]() | CELLULASE![]() | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Baumann, M.J. / Eklof, J.M. / Michel, G. / Kallas, A. / Teeri, T.T. / Brumer, H. / Czjzek, M. | ||||||
![]() | ![]() Title: Structural Evidence for the Evolution of Xyloglucanase Activity from Xyloglucan Endo-Transglycosylases: Biological Implications for Cell Wall Metabolism. Authors: Baumann, M.J. / Eklof, J.M. / Michel, G. / Kallas, A.M. / Teeri, T.T. / Czjzek, M. / Brumer, H. | ||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 124.4 KB | Display | ![]() |
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PDB format | ![]() | 97.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2uwaC ![]() 2uwbC ![]() 1un1S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Ens-ID: 1 / Refine code: 5
NCS oper: (Code: given Matrix: (0.49921, -0.86648, 0.00206), Vector ![]() |
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Components
#1: Protein | ![]() Mass: 30916.084 Da / Num. of mol.: 2 / Fragment: RESIDUES 25-295 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: Q07524, ![]() ![]() #2: Water | ChemComp-HOH / | ![]() Sequence details | THE FIRST TWO RESIDUES ARE NOT DEFINED BY THE ELECTRON DENSITY | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 4.5 Å3/Da / Density % sol: 72.7 % |
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Crystal grow![]() | pH: 8.5 Details: 2-3 M POTASSIUM ACETATE, 2% 2, 5-METHYLPENTANE-DIOL AND 100 MM TRIS BUFFER AT PH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Mar 21, 2006 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 2.3→45 Å / Num. obs: 47776 / % possible obs: 98.3 % / Observed criterion σ(I): 0 / Redundancy: 4.7 % / Rmerge(I) obs: 0.11 / Net I/σ(I): 10.9 |
Reflection shell | Resolution: 2.3→2.42 Å / Redundancy: 4.7 % / Rmerge(I) obs: 0.6 / Mean I/σ(I) obs: 2.5 / % possible all: 99.1 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1UN1 Resolution: 2.3→31.31 Å / Cor.coef. Fo:Fc: 0.959 / Cor.coef. Fo:Fc free: 0.946 / SU B: 8.172 / SU ML: 0.105 / Cross valid method: THROUGHOUT / ESU R: 0.162 / ESU R Free: 0.147 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.24 Å2
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Refinement step | Cycle: LAST / Resolution: 2.3→31.31 Å
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Refine LS restraints |
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