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Yorodumi- PDB-2rqm: NMR Solution Structure of Mesoderm Development (MESD) - open conf... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2rqm | ||||||
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Title | NMR Solution Structure of Mesoderm Development (MESD) - open conformation | ||||||
Components | Mesoderm development candidate 2 | ||||||
Keywords | CHAPERONE | ||||||
Function / homology | Function and homology information positive regulation of skeletal muscle acetylcholine-gated channel clustering / protein localization to cell surface / low-density lipoprotein particle receptor binding / mesoderm development / phagocytosis / ossification / Wnt signaling pathway / protein folding / endoplasmic reticulum / identical protein binding / plasma membrane Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Model details | D45-K184 Conserved Core Region | ||||||
Authors | Koehler, C. / Lighthouse, J.K. / Werther, T. / Andersen, O.M. / Diehl, A. / Schmieder, P. / Holdener, B.C. / Oschkinat, H. | ||||||
Citation | Journal: Structure / Year: 2011 Title: The Structure of MESD45-184 Brings Light into the Mechanism of LDLR Family Folding Authors: Kohler, C. / Lighthouse, J.K. / Werther, T. / Andersen, O.M. / Diehl, A. / Schmieder, P. / Du, J. / Holdener, B.C. / Oschkinat, H. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rqm.cif.gz | 867.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rqm.ent.gz | 732.7 KB | Display | PDB format |
PDBx/mmJSON format | 2rqm.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rq/2rqm ftp://data.pdbj.org/pub/pdb/validation_reports/rq/2rqm | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 16148.112 Da / Num. of mol.: 1 Fragment: D45-K184 conserved core region, UNP residues 45-184 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Mesdc2 / Plasmid: pET-30 Xa/LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9ERE7 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR / Details: D45-K184 Conserved Core Region | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | pH: 5.5 / Pressure: ambient / Temperature: 300 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | ||||||||||||||||||||||||||||||||||||||||
NMR constraints | NOE constraints total: 527 / NOE intraresidue total count: 0 / NOE long range total count: 147 / NOE medium range total count: 107 / NOE sequential total count: 273 / Hydrogen bond constraints total count: 106 / Protein chi angle constraints total count: 0 / Protein other angle constraints total count: 0 / Protein phi angle constraints total count: 113 / Protein psi angle constraints total count: 113 | ||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 1 |