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Yorodumi- PDB-2rpp: Solution structure of Tandem zinc finger domain 12 in Muscleblind... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2rpp | ||||||
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Title | Solution structure of Tandem zinc finger domain 12 in Muscleblind-like protein 2 | ||||||
Components | Muscleblind-like protein 2 | ||||||
Keywords | TRANSCRIPTION / ZINC FINGER DOMAIN / C3H / Alternative splicing / Cytoplasm / Metal-binding / Nucleus / RNA-binding / Zinc / Zinc-finger / structural genomics / NPPSFA / National Project on Protein Structural and Functional Analyses / RIKEN Structural Genomics/Proteomics Initiative / RSGI | ||||||
Function / homology | Function and homology information regulation of RNA splicing / RNA splicing / mRNA processing / sequence-specific double-stranded DNA binding / RNA binding / nucleoplasm / metal ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | SOLUTION NMR / torsion angle dynamics, restrained MOLECULAR DYNAMICS | ||||||
Authors | Abe, C. / Dang, W. / Tsuda, K. / Muto, Y. / Inoue, M. / Kigawa, T. / Terada, T. / Shirouzu, M. / Yokoyama, S. / RIKEN Structural Genomics/Proteomics Initiative (RSGI) | ||||||
Citation | Journal: Protein Sci. / Year: 2009 Title: Solution structure of the RNA binding domain in the human muscleblind-like protein 2 Authors: He, F. / Dang, W. / Abe, C. / Tsuda, K. / Inoue, M. / Watanabe, S. / Kobayashi, N. / Kigawa, T. / Matsuda, T. / Yabuki, T. / Aoki, M. / Seki, E. / Harada, T. / Tomabechi, Y. / Terada, T. / ...Authors: He, F. / Dang, W. / Abe, C. / Tsuda, K. / Inoue, M. / Watanabe, S. / Kobayashi, N. / Kigawa, T. / Matsuda, T. / Yabuki, T. / Aoki, M. / Seki, E. / Harada, T. / Tomabechi, Y. / Terada, T. / Shirouzu, M. / Tanaka, A. / Guntert, P. / Muto, Y. / Yokoyama, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2rpp.cif.gz | 528.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2rpp.ent.gz | 439.6 KB | Display | PDB format |
PDBx/mmJSON format | 2rpp.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rp/2rpp ftp://data.pdbj.org/pub/pdb/validation_reports/rp/2rpp | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9829.062 Da / Num. of mol.: 1 / Fragment: ZINC FINGER DOMAIN, UNP residues 7-82 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: MBNL2, MBLL, MBLL39, MLP1 / Plasmid: P050404-08 / Production host: CELL-FREE SYNTHESIS (others) / References: UniProt: Q5VZF2 |
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#2: Chemical |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1.21 mM [U-100% 13C; U-100% 15N] MUSCLEBLIND-LIKE PROTEIN 2, 90% H2O/10% D2O Solvent system: 90% H2O/10% D2O |
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Sample | Conc.: 1.21 mM / Component: MUSCLEBLIND-LIKE PROTEIN 2 / Isotopic labeling: [U-100% 13C; U-100% 15N] |
Sample conditions | Ionic strength: 120 / pH: 7.0 / Pressure: AMBIENT / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: BRUKER AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 800 MHz |
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-Processing
NMR software |
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Refinement | Method: torsion angle dynamics, restrained MOLECULAR DYNAMICS Software ordinal: 1 | ||||||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: target function / Conformers calculated total number: 100 / Conformers submitted total number: 20 / Representative conformer: 1 |