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Yorodumi- PDB-2pps: PHOTOSYNTHETIC REACTION CENTER AND CORE ANTENNA SYSTEM (TRIMERIC)... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2pps | ||||||||||||
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Title | PHOTOSYNTHETIC REACTION CENTER AND CORE ANTENNA SYSTEM (TRIMERIC), ALPHA CARBON ONLY | ||||||||||||
Components | (PHOTOSYSTEM I) x 6 | ||||||||||||
Keywords | PHOTOSYNTHESIS / PHOTOSYNTHETIC REACTION CENTER / OXYGENIC PHOTOSYNTHESIS / CORE-ANTENNA LIGHT-HARVESTING SYSTEM / THERMOPHILIC CYANOBACTERIUM / HELIX-BUNDLE MEMBRANE PROTEIN | ||||||||||||
Function / homology | CHLOROPHYLL A / PHYLLOQUINONE / IRON/SULFUR CLUSTER Function and homology information | ||||||||||||
Biological species | Synechococcus elongatus (bacteria) | ||||||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 4 Å | ||||||||||||
Authors | Krauss, N. / Schubert, W.-D. / Klukas, O. / Fromme, P. / Witt, H.T. / Saenger, W. | ||||||||||||
Citation | Journal: Nat.Struct.Biol. / Year: 1996 Title: Photosystem I at 4 A resolution represents the first structural model of a joint photosynthetic reaction centre and core antenna system. Authors: Krauss, N. / Schubert, W.D. / Klukas, O. / Fromme, P. / Witt, H.T. / Saenger, W. #1: Journal: J.Mol.Biol. / Year: 1997 Title: Photosystem I of Synechococcus Elongatus at 4 A Resolution: Comprehensive Structure Analysis Authors: Schubert, W.D. / Klukas, O. / Krauss, N. / Saenger, W. / Fromme, P. / Witt, H.T. #2: Journal: Photosynthesis : From Light to Biosphere : Proceedings of the Xth International Photosynthesis Congress, Montpellier, France, 20-25 August 1995 Year: 1995 Title: Present State of the Crystal Structure Analysis of Photosystem I at 4.5 A Resolution Authors: Schubert, W.D. / Klukas, O. / Krauss, N. / Saenger, W. / Fromme, P. / Witt, H.T. #3: Journal: Nature / Year: 1993 Title: Three-Dimensional Structure of System I of Photosynthesis at 6A Resolution, Authors: Krauss, N. / Hinrichs, W. / Witt, I. / Fromme, P. / Pritzkow, W. / Dauter, Z. / Betzel, C. / Wilson, K.S. / Witt, H.T. / Saenger, W. #4: Journal: Ber.Bunsenges.Phys.Chem. / Year: 1988 Title: X-Ray Characterization of Single Crystals of the Reaction Center I of Water Splitting Photosynthesis Authors: Witt, I. / Witt, H.T. / Di Fiore, D. / Rogner, M. / Hinrichs, W. / Saenger, W. / Granzin, J. / Betzel, C. / Dauter, Z. | ||||||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2pps.cif.gz | 120.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2pps.ent.gz | 83.2 KB | Display | PDB format |
PDBx/mmJSON format | 2pps.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/pp/2pps ftp://data.pdbj.org/pub/pdb/validation_reports/pp/2pps | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
-Protein , 6 types, 6 molecules ABLKFC
#1: Protein | Mass: 40698.164 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
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#2: Protein | Mass: 42825.812 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
#3: Protein | Mass: 9464.658 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
#4: Protein | Mass: 5464.728 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
#5: Protein | Mass: 11081.651 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
#6: Protein | Mass: 6826.406 Da / Num. of mol.: 1 Fragment: THE PSI-PROTOMER IS A COMPLEX OF PSAA, PSAB, PSAC, PSAD, PSAE, PSAF, PSAI, PSAJ, PSAK, PSAL, PSAM Source method: isolated from a natural source / Source: (natural) Synechococcus elongatus (bacteria) / Cellular location: THYLAKOID MEMBRANEThylakoid |
-Non-polymers , 3 types, 93 molecules
#7: Chemical | ChemComp-CLA / #8: Chemical | ChemComp-PQN / | #9: Chemical | |
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-Details
Compound details | CHAIN A COMPRISES ONE OF THE TWO LARGE, CENTRAL, MEMBRANE INTEGRAL SUBUNITS OF THE REACTION CENTER ...CHAIN A COMPRISES ONE OF THE TWO LARGE, CENTRAL, MEMBRANE INTEGRAL SUBUNITS OF THE REACTION CENTER OF PSI - EITHER PSAA OR PSAB. CHAIN B COMPRISES THE SECOND OF THE TWO LARGE, CENTRAL, MEMBRANE INTEGRAL SUBUNITS OF THE REACTION CENTER OF PSI - EITHER PSAB OR PSAA. CHAIN C COMPRISES THE MODEL OF THE STROMAL EXTRINSIC SUBUNIT OF PSI - PSAC. CHAIN F COMPRISES THOSE SECTIONS OF THE PRESENT MODEL LOCATED DISTAL TO THE TRIMERIZAT |
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Nonpolymer details | THE ATOM NAMING OF THE CHLOROPHYLL MOLECULES IS ARBITRARY IN THE SENSE THAT THE AUTHORS DO NOT WISH ...THE ATOM NAMING OF THE CHLOROPHYL |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density % sol: 80 % | ||||||||||||||||||||||||
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Crystal grow | pH: 6.4 Details: PROTEIN CONCENTRATION:80 MG/ML METHOD: MICRODIALYSIS BUFFER: 0.02% BETA-DODECYLMALTOSIDE, 5 MM MES, PH 6.4 | ||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 4 ℃ / Method: unknown | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 279 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, HAMBURG / Beamline: X11 / Wavelength: 0.92 |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 1, 1993 / Details: FLAT SEGMENTED MIRROR |
Radiation | Monochromator: BENT CYLINDRICAL GE(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.92 Å / Relative weight: 1 |
Reflection | Resolution: 3.85→34.9 Å / Num. obs: 69153 / % possible obs: 94.4 % / Observed criterion σ(I): 3 / Redundancy: 2.8 % / Rmerge(I) obs: 0.085 / Rsym value: 0.102 / Net I/σ(I): 6.4 |
Reflection shell | Resolution: 3.85→3.95 Å / Redundancy: 2 % / Mean I/σ(I) obs: 3.6 / Rsym value: 0.173 / % possible all: 81.3 |
Reflection shell | *PLUS % possible obs: 81.3 % / Rmerge(I) obs: 0.173 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Highest resolution: 4 Å Details: MODEL STRUCTURE IS BASED ON 4.0 ANGSTROM MIR MAP. THERE WAS NO REFINEMENT DUE TO LIMITED RESOLUTION. | |||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 4 Å
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