+Open data
-Basic information
Entry | Database: PDB / ID: 2oyv | |||||||||
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Title | Neurotensin in DPC micelles | |||||||||
Components | neurotensin | |||||||||
Keywords | NEUROPEPTIDE / type I turn | |||||||||
Function / homology | Function and homology information neuropeptide receptor binding / neuropeptide hormone activity / neuropeptide signaling pathway / axon terminus / transport vesicle / blood vessel diameter maintenance / Peptide ligand-binding receptors / positive regulation of NF-kappaB transcription factor activity / G alpha (q) signalling events / receptor ligand activity ...neuropeptide receptor binding / neuropeptide hormone activity / neuropeptide signaling pathway / axon terminus / transport vesicle / blood vessel diameter maintenance / Peptide ligand-binding receptors / positive regulation of NF-kappaB transcription factor activity / G alpha (q) signalling events / receptor ligand activity / positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction / negative regulation of gene expression / intracellular membrane-bounded organelle / positive regulation of gene expression / signal transduction / extracellular region Similarity search - Function | |||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||
Authors | Monti, J.P. / Coutant, J. / Curmi, P.A. | |||||||||
Citation | Journal: Biochemistry / Year: 2007 Title: NMR Solution Structure of Neurotensin in Membrane-Mimetic Environments: Molecular Basis for Neurotensin Receptor Recognition. Authors: Coutant, J. / Curmi, P.A. / Toma, F. / Monti, J.P. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2oyv.cif.gz | 63.8 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2oyv.ent.gz | 49.9 KB | Display | PDB format |
PDBx/mmJSON format | 2oyv.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oy/2oyv ftp://data.pdbj.org/pub/pdb/validation_reports/oy/2oyv | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 1675.948 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: This sequence occurs naturally in humans. / References: UniProt: P30990*PLUS |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR experiment | Type: 2D NOESY |
NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details | Contents: 5 mM neurotensin, 30 mg DPC, H2O:D2O 12:1, pH 6.4 / Solvent system: H2O:D2O (12:1) pH = 6.4 |
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Sample conditions | pH: 6.4 / Pressure: 1 atm / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 600 MHz |
-Processing
NMR software | Name: Discover / Classification: refinement |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: the structures are based on a total of 204 NOE-derived distance constraints. |
NMR representative | Selection criteria: closest to the average |
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 14 |