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- PDB-2o1h: Naturally occurring mutation of Humna ABO(H) Galactosyltransferas... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2o1h | ||||||
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Title | Naturally occurring mutation of Humna ABO(H) Galactosyltransferase in complex with UDP: GTB/M214T_UDP | ||||||
![]() | ABO glycosyltransferase | ||||||
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Function / homology | ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Letts, J.A. / Borisova, S.N. / Evans, S.V. | ||||||
![]() | ![]() Title: Structural effects of naturally occurring human blood group B galactosyltransferase mutations adjacent to the DXD motif. Authors: Persson, M. / Letts, J.A. / Hosseini-Maaf, B. / Borisova, S.N. / Palcic, M.M. / Evans, S.V. / Olsson, M.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 78.2 KB | Display | ![]() |
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PDB format | ![]() | 55.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 2o1fC ![]() 2o1gC ![]() 1lz7S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 34496.754 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() | ||||||
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#2: Chemical | ChemComp-HG / ![]() #3: Chemical | ChemComp-MN / | #4: Chemical | ChemComp-UDP / | ![]() #5: Water | ChemComp-HOH / | ![]() |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.08 % |
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Crystal grow![]() | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: ADA, manganese chloride, ammonium sulfate, MPD, glycerol, PEG 4000,, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 113 K |
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Diffraction source | Source: ![]() |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Apr 27, 2006 |
Radiation | Monochromator: Osmic "Blue" confocal x-ray mirrors with power levels of 30 watts Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.67→20 Å / Num. all: 36659 / Num. obs: 36659 / % possible obs: 100 % / Redundancy: 6.71 % / Rmerge(I) obs: 0.054 / Net I/σ(I): 15.2 |
Reflection shell | Resolution: 1.67→1.73 Å / Redundancy: 6.33 % / Rmerge(I) obs: 0.299 / Mean I/σ(I) obs: 4.9 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: 1lz7 Resolution: 1.67→20 Å / Cor.coef. Fo:Fc: 0.966 / Cor.coef. Fo:Fc free: 0.945 / SU B: 1.637 / SU ML: 0.057 / Cross valid method: THROUGHOUT / ESU R: 0.093 / ESU R Free: 0.1 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.584 Å2
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Refinement step | Cycle: LAST / Resolution: 1.67→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.67→1.713 Å / Total num. of bins used: 20
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