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Yorodumi- PDB-2n7c: Solution structure of Plasmodium falciparum SR1-RRM1 in complex w... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2n7c | ||||||
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Title | Solution structure of Plasmodium falciparum SR1-RRM1 in complex with ACAUCA RNA | ||||||
Components |
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Keywords | RNA Binding Protein/RNA / Serine/Arginine rich protein / RNA Binding Protein-RNA complex | ||||||
Function / homology | Function and homology information RNA splicing / spliceosomal complex / mRNA processing / ribonucleoprotein complex / mRNA binding / nucleus / cytoplasm Similarity search - Function | ||||||
Biological species | Plasmodium falciparum 3D7 (eukaryote) synthetic construct (others) | ||||||
Method | SOLUTION NMR / DGSA-distance geometry simulated annealing, simulated annealing | ||||||
Model details | closest to the average, model8 | ||||||
Authors | Ganguly, A.K. / Verma, G. / Bhavesh, N.S. | ||||||
Citation | Journal: J. Mol. Biol. / Year: 2019 Title: The N-terminal RNA Recognition Motif of PfSR1 Confers Semi-specificity for Pyrimidines during RNA Recognition. Authors: Ganguly, A.K. / Verma, G. / Bhavesh, N.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2n7c.cif.gz | 609.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2n7c.ent.gz | 513 KB | Display | PDB format |
PDBx/mmJSON format | 2n7c.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n7/2n7c ftp://data.pdbj.org/pub/pdb/validation_reports/n7/2n7c | HTTPS FTP |
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-Related structure data
Related structure data | 2n3lC C: citing same article (ref.) |
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Similar structure data | |
Other databases |
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-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 9997.198 Da / Num. of mol.: 1 / Fragment: UNP residues 1-86 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Plasmodium falciparum 3D7 (eukaryote) / Strain: 3D7 / Gene: PFE0865c / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): CodonPlus / References: UniProt: Q8I3T5 |
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#2: RNA chain | Mass: 1859.189 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 125 / pH: 5.9 / Pressure: ambient / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: DGSA-distance geometry simulated annealing, simulated annealing Software ordinal: 1 / Details: Refinement in explicit solvent | ||||||||||||||||||||||||||||||||||||||||||||
NMR representative | Selection criteria: closest to the average | ||||||||||||||||||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations Conformers calculated total number: 200 / Conformers submitted total number: 20 / Representative conformer: 8 |